CAJ | 학술논문

目的：应用巢式PCR方法与环介导等温扩增（LAM P）方法检测蜱中莱姆病螺旋体带菌率。方法从青海省循化县与辽宁省新宾县共采集蜱112只，采用巢式PCR与LAM P两种方法检测蜱的莱姆病螺旋体带菌率。结果采用两种方法检测青海循化蜱标本51份，12份阳性，阳性率为23．53％；辽宁省新宾县蜱标本61份，18份阳性，阳性率为29．51％。采用巢式PCR方法检测112份蜱标本，20份阳性，阳性率为17．86％；采用LAM P方法检测112份蜱标本，15份阳性，阳性率为13．39％，两种方法差异无统计学意义（χ2＝0．85，P＞0．05）。结论巢式PCR与LAMP方法均可用于蜱标本莱姆病螺旋体的检测，两种方法共同使用可以提高莱姆病螺旋体的检出率。同时，本研究首次报道了青海循化县和辽宁新宾县蜱的莱姆病螺旋体带菌率，完善了我国高林区覆盖率地区的蜱媒带菌率数据。
목적：응용소식PCR방법여배개도등온확증（LAM P）방법검측비중래모병라선체대균솔。방법종청해성순화현여요녕성신빈현공채집비112지，채용소식PCR여LAM P량충방법검측비적래모병라선체대균솔。결과채용량충방법검측청해순화비표본51빈，12빈양성，양성솔위23．53％；요녕성신빈현비표본61빈，18빈양성，양성솔위29．51％。채용소식PCR방법검측112빈비표본，20빈양성，양성솔위17．86％；채용LAM P방법검측112빈비표본，15빈양성，양성솔위13．39％，량충방법차이무통계학의의（χ2＝0．85，P＞0．05）。결론소식PCR여LAMP방법균가용우비표본래모병라선체적검측，량충방법공동사용가이제고래모병라선체적검출솔。동시，본연구수차보도료청해순화현화료녕신빈현비적래모병라선체대균솔，완선료아국고림구복개솔지구적비매대균솔수거。
ABSTRACT:Nested‐PCR and loop‐mediated isothermal amplification (LAMP) were applied to identify the Borrelia burg‐dor f eri (B .burgdor f eri) in ticks in this study .A total of 112 adult ixodes were collected from vegetation in a forest area and farm animals in Xunhua County ,Qinghai Province and Xinbin County ,Liaoning Province .The ticks were examined for the presence of B .burgdorferi by nested‐PCR and LAMP .Results showed that 12 in 51 samples were found positive in Xunhua County (23 .53% ) .While positive rate in Xinbin County was 29 .51% with 18 samples positive in 61 samples .In total of 112 tick samples ,the PCR‐positive rate was 17 .86% with 20 positive samples identified ,whereas 15 positive samples were con‐firmed with positive rate of 13 .39 % by LAMP assay .There was no significant difference between the two assays (Х2 =0 .85 , P>0 .05) .Results suggest that both nested‐PCR and LAMP could be used in identifying B .burgdorferi in ticks .Combina‐tion of these two assays could improve the testing results .This is the first report of B .burgdorferi in ticks in Xunhua and Xinbin counties ,and helps to complete the database of the infection rate of B .burgdor f eri in ticks in the widely‐forested area of China .