CAJ | 학술논문

目的检测乳腺癌患者血清胸苷激酶1(Thymidine Kinase 1,TK1)水平,探讨手术对乳腺癌患者血清TK1水平的影响.方法采用免疫印迹-增强化学发光法检测33例正常人及65例乳腺癌患者血清中TK1含量,分析乳腺癌患者血清TK1水平与临床病理特征的关系,比较38例乳腺癌患者手术前后血清TK1水平.结果乳腺癌患者相对于正常对照组血清TK1水平明显升高,两者比较差异有统计学意义(P＜0.01);血清TK1水平与患者年龄、病理类型无相关;血清TK1水平与乳腺癌患者临床分期有关,Ⅳ期乳腺癌血清TK1水平明显高于Ⅲ期,Ⅲ期明显高于Ⅱ期,Ⅱ期明显高于Ⅰ期,两两比较差异有统计学意义(P＜0.01,P＜0.05);乳腺癌患者术后血清TK1水平明显低于术前,两者比较差异有统计学意义(P＜0.01).结论血清TK1水平在乳腺癌患者血清中显著增加,血清TK1表达水平可能可以作为反映乳腺癌患者病情、疗效判断和预后评价的一个参考指标.
목적 검측유선암환자혈청흉감격매1(Thymidine Kinase 1,TK1)수평,탐토수술대유선암환자혈청TK1수평적영향.방법 채용면역인적-증강화학발광법검측33례정상인급65례유선암환자혈청중TK1함량,분석유선암환자혈청TK1수평여림상병리특정적관계,비교38례유선암환자수술전후혈청TK1수평.결과 유선암환자상대우정상대조조혈청TK1수평명현승고,량자비교차이유통계학의의(P＜0.01);혈청TK1수평여환자년령、병리류형무상관;혈청TK1수평여유선암환자림상분기유관,Ⅳ기유선암혈청TK1수평명현고우Ⅲ기,Ⅲ기명현고우Ⅱ기,Ⅱ기명현고우Ⅰ기,량량비교차이유통계학의의(P＜0.01,P＜0.05);유선암환자술후혈청TK1수평명현저우술전,량자비교차이유통계학의의(P＜0.01).결론 혈청TK1수평재유선암환자혈청중현저증가,혈청TK1표체수평가능가이작위반영유선암환자병정、료효판단화예후평개적일개삼고지표.
Objective To investigate the influence of surgery on the level of thymidine kinase 1(TK1) in the serum of patients with breast carcinoma detecting its serum level.Methods Sensitive chemiluminescence dot-blot assay was used to detect the serum TK1 levels of 33 healthy women and 65patients with breast carcinoma.The clinical implications were also analyzed.The serum TK1 levels of 38 patients with breast carcinoma were compared between before and after the operatiou.Results The serum TK1 level was higher in the patients with breast carcinoma than in the healthy women,with a statistical difference(P ＜ 0.01).Serum TK1 level didn' t correlated with age and pathologic types but correlated with clinical stage; the serum TK1 level was higher in stage Ⅳ patients than in stage Ⅲ,in stage Ⅲ than in stage Ⅱ,and in stage Ⅱ than in stage Ⅰ (P ＜ 0.01,P ＜ 0.05).The level of serum TK1 was obviously reduced in patients with breast carcinoma after the operation(P ＜ 0.01).Conclusions The serum TK1 levels in patients with breast carcinoma are higher; it could be an indicator assessing the disease status,therapeutic effect,and the prognosis of breast carcinoma.