CAJ | 학술논문

目的：探讨丹皮酚对烫伤诱导小鼠肝损伤肝组织自噬蛋白LC3表达的影响。方法小鼠随机分为空白组、模型组和丹皮酚组，用90℃热水建立烫伤模型，丹皮酚组小鼠在建模前1 h用丹皮酚溶液（60 mg· kg－1）灌胃。观察建模后第2，6，12，24 h小鼠血清丙氨酸氨基转移酶（ ALT）与血清天门冬氨酸氨基转移酶（ AST）水平变化及肝组织LC3表达情况。结果丹皮酚可有效降低小鼠血清ALT、AST水平，与模型组比较差异有统计学意义，同时可以使肝组织LC3表达增加（均P＜0.05）。结论丹皮酚通过增加自噬蛋白LC3表达对深Ⅱ度烫伤诱导的肝损伤具有保护作用。
목적：탐토단피분대탕상유도소서간손상간조직자서단백LC3표체적영향。방법소서수궤분위공백조、모형조화단피분조，용90℃열수건립탕상모형，단피분조소서재건모전1 h용단피분용액（60 mg· kg－1）관위。관찰건모후제2，6，12，24 h소서혈청병안산안기전이매（ ALT）여혈청천문동안산안기전이매（ AST）수평변화급간조직LC3표체정황。결과단피분가유효강저소서혈청ALT、AST수평，여모형조비교차이유통계학의의，동시가이사간조직LC3표체증가（균P＜0.05）。결론단피분통과증가자서단백LC3표체대심Ⅱ도탕상유도적간손상구유보호작용。
Objective To explore the effect of Paeonol on LC 3 expre-ssion of liver tissues in mice with liver injury induced by deep partial thickness burns wound .Methods Mice were randomly divided into con-trol group, model group and Paeonol group .The deep partial thickness burns wound model was made by 90 ℃ water , and the Paeonol group were intragastric administration treatment of Paeonol solution ( 60 mg· kg-1 ).The levels of alanine aminotransferase (ALT) and aspartate aminotransferase ( AST ) in the serum were respectively measured and LC3 expression of liver tissue was observed between 2 h,6 h,12 h,24 h after model established .Results The levels of ALT and AST in the serum of Paeonol group reduced obviously .Compared Paeonol group with model group , the differences were statistically significant ( P <0.05 ) . Meanwhile , Paeonol could induce LC 3 expression of liver tissues .The effects of high -dosage group and medium - dosage group were especially conspicuous .Conclusion Paeonol has better protective effect on liver injury induced with deep Partial thickness burns wound , the mechanism of which is mainly related with inducing LC 3 expression and inhibiting apoptosis of the liver cells .