CAJ | 학술논문

目的：探讨2－十二烯酸对人肝癌细胞的毒性作用。方法分别给予人肝癌细胞3个浓度（0.1，0.3，0.9 mg· L －1）2－十二烯酸，培养6，12，18，24，48 h，用台盼蓝法、MTT法、流式细胞仪检测2－十二烯酸对人肿瘤细胞的毒性作用。结果培养48 h，3个浓度的2－十二烯酸对人肝癌细胞增殖抑制率分别为45.46％，7.16％，9.18％，与对照组比差异有统计学意义（ P﹤0.01）；凋亡率分别为14.8％，20.2％，24.2％。结论2－十二烯酸对人肝癌细胞增殖有明显的毒性作用。
목적：탐토2－십이희산대인간암세포적독성작용。방법분별급여인간암세포3개농도（0.1，0.3，0.9 mg· L －1）2－십이희산，배양6，12，18，24，48 h，용태반람법、MTT법、류식세포의검측2－십이희산대인종류세포적독성작용。결과배양48 h，3개농도적2－십이희산대인간암세포증식억제솔분별위45.46％，7.16％，9.18％，여대조조비차이유통계학의의（ P﹤0.01）；조망솔분별위14.8％，20.2％，24.2％。결론2－십이희산대인간암세포증식유명현적독성작용。
Objective To investigate the toxicity of 2-dodecenoic acid on human hepatocarcinoma cells.Methods The present study is to in-vestigate the toxic effects of 2 -dodecenoic acid on human hepatoma tumor cells by using trypan blue staining , MTT assay , and flow cytometry analysis.Results After cancer cells were treated with concentrations of 0.1 , 0.3 ,0.9 mg· L-1 2-dodecenoic acid at 6 h, 12 h, 18 h, 24 h, and 48 h, respectively.Compared to the control group , 48 h after treat-ment, the inhibition rates of the hepatoma cells proliferation in treatment group were increased by 45.46%, 67.16%, 79.18%at the above three concentrations , respectively ( P<0.01 ).Flow cytometry analysis showed that the apoptosis rates after the 2-dodecenoic acid treatment were in-creased by 14.8%, 20.2% and 24.2% over the control group.Conclusion These results indicated that the 2-dodecenoic acid has a significant toxic effect on human hepatoma cells.