中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2014年
12期
1096-1097,1104
,共3页
代强%薛峰%王似锦%高春%郑波
代彊%薛峰%王似錦%高春%鄭波
대강%설봉%왕사금%고춘%정파
VanC型肠球菌%API%VITEK2%万古霉素耐药%动力实验
VanC型腸毬菌%API%VITEK2%萬古黴素耐藥%動力實驗
VanC형장구균%API%VITEK2%만고매소내약%동력실험
VanC type Enterococcus%API%VITEK2%vancomycin resis-tant%motility test
目的:比较API和VITEK2法鉴别VanC型肠球菌的准确性。方法针对2005~2011年收集的50株VanC型肠球菌,采用API和VITEK2方法进行种属鉴定,结合细菌动力实验,用分子生物学方法做对照,以判断API和VITEK2鉴定结果的准确性。结果 API和VITEK2方法分别筛选出VanC型肠球菌21,15株,与分子生物学方法比较,其准确率分别为28.6%,86.7%,结合动力实验结果后,其准确率均达到100%。结论 VITEK2比API在诊断VanC型肠球菌时具有更好的准确性,2种方法联合动力实验可为临床提供准确的细菌学诊断。
目的:比較API和VITEK2法鑒彆VanC型腸毬菌的準確性。方法針對2005~2011年收集的50株VanC型腸毬菌,採用API和VITEK2方法進行種屬鑒定,結閤細菌動力實驗,用分子生物學方法做對照,以判斷API和VITEK2鑒定結果的準確性。結果 API和VITEK2方法分彆篩選齣VanC型腸毬菌21,15株,與分子生物學方法比較,其準確率分彆為28.6%,86.7%,結閤動力實驗結果後,其準確率均達到100%。結論 VITEK2比API在診斷VanC型腸毬菌時具有更好的準確性,2種方法聯閤動力實驗可為臨床提供準確的細菌學診斷。
목적:비교API화VITEK2법감별VanC형장구균적준학성。방법침대2005~2011년수집적50주VanC형장구균,채용API화VITEK2방법진행충속감정,결합세균동력실험,용분자생물학방법주대조,이판단API화VITEK2감정결과적준학성。결과 API화VITEK2방법분별사선출VanC형장구균21,15주,여분자생물학방법비교,기준학솔분별위28.6%,86.7%,결합동력실험결과후,기준학솔균체도100%。결론 VITEK2비API재진단VanC형장구균시구유경호적준학성,2충방법연합동력실험가위림상제공준학적세균학진단。
Objective To compare the API and VITEK2 in identifying VanC type Enterococcus.Method A total of 50 VanC type Entero-coccus isolates from 2005~2011 were investigated.We performed the API and VITEK2 test to identified the VanC type Enterococcus respec-tively.API and VITEK2 results combining with the motility test was compared with molecular biology methods to determine the accuracy of API and VITEK2.The MIC of vancomycin and tecoplanin for the strains was performed by agar dilution method.Results Twenty -one and 15 strains of VanC phenotype Enterococcus were identified by the API and VITEK2 test respectively.Compared with molecular bio-logy methods, the accuracy rate was 28.6% and 86.7% respective-ly.Combined with motility test, the accuracy rate of API and VITEK2 reached 100%.Conclusion The VITEK2 had a higher accuracy rate in identifying VanC phenotype Enterococcus than API.API/VITEK2 method combined with bacterial motility test can provide an accurate bacteriological diagnosis for the patients.