CAJ | 학술논문

采用ISSR分子标记对陆川猪及其肉制品进行鉴伪识别，利用ISSR技术，对纯种陆川猪、陆川猪杂交品种（二元杂猪和三元杂猪）及广西本地的巴马香猪和环江香猪的DNA指纹图谱进行分析。结果表明：从34条引物中筛选出3条带型清晰、重复性好的引物WM12、WM27、WM10，其中引物WM12可用于鉴别陆川猪、二元杂猪和三元杂猪的生鲜样品及腊肉制品，引物WM27可用于鉴别陆川猪和巴马香猪的生鲜样品及腊肉制品，引物WM10用可于鉴别陆川猪和环江香猪的生鲜样品及腊肉制品。ISSR技术可快速准确的鉴定陆川猪、陆川猪杂交品种、巴马香猪、环江香猪及其肉制品。
채용ISSR분자표기대륙천저급기육제품진행감위식별，이용ISSR기술，대순충륙천저、륙천저잡교품충（이원잡저화삼원잡저）급엄서본지적파마향저화배강향저적DNA지문도보진행분석。결과표명：종34조인물중사선출3조대형청석、중복성호적인물WM12、WM27、WM10，기중인물WM12가용우감별륙천저、이원잡저화삼원잡저적생선양품급석육제품，인물WM27가용우감별륙천저화파마향저적생선양품급석육제품，인물WM10용가우감별륙천저화배강향저적생선양품급석육제품。ISSR기술가쾌속준학적감정륙천저、륙천저잡교품충、파마향저、배강향저급기육제품。
In this study, species identification of Luchuan pig meat and meat products was carried out by DNA fingerprint profiling of meats from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs using ISSR (inter-simple sequence repeat) molecular markers. The results showed that 3 clear and repeatable primer pairs WM12, WM27 and WM10 were selected from 34 primers. WM12 was useful for discriminating fresh and cured meat from purebred Luchuan and hybrid Luchuan pigs, while Luchuan pigs could be distinguished from Bama miniature pigs by using WM27, and from Huangjiang miniature pigs by using WM10. In conclusion, the ISSR method enables rapid and accurate identification of fresh meat and meat products from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs.