CAJ | 학술논문

目的：采用星点设计－响应面分析法优化陕产铁筷子皂苷的醇提取工艺。方法：选用乙醇为提取溶剂，以乙醇浓度、乙醇倍数、提取时间为自变量，铁筷子皂苷含量为因变量，采用响应面分析法优化该药材的提取工艺参数。结果：确定了提取工艺的最佳条件为：乙醇浓度70％，提取时间2 h，乙醇倍数6.5倍。在此条件下铁筷子提取物中皂苷的含量为0.0658 g／g，二项式拟合复相关系数 R2＝0.8475。结论：该工艺方法能够用于陕产铁筷子皂苷的提取，并且具有简单、可行，预测性较好优势。
목적：채용성점설계－향응면분석법우화협산철쾌자조감적순제취공예。방법：선용을순위제취용제，이을순농도、을순배수、제취시간위자변량，철쾌자조감함량위인변량，채용향응면분석법우화해약재적제취공예삼수。결과：학정료제취공예적최가조건위：을순농도70％，제취시간2 h，을순배수6.5배。재차조건하철쾌자제취물중조감적함량위0.0658 g／g，이항식의합복상관계수 R2＝0.8475。결론：해공예방법능구용우협산철쾌자조감적제취，병차구유간단、가행，예측성교호우세。
Objective:To optimize the extraction technology of total dioscin from Helleborus thibetanus Franch of Shaanxi by central composite design response surface method.Methods:Ethanol was used as the extraction solvent,and the parameters of ex-traction tech-nology of total dioscin from Helleborus thibetanus Franch were optimized by 3 factors and 5 levels.Independent variables were ethanol concentration,ethanol multiples and extraction time;dependent variable was the dioscin content.Results:The optimal extraction technol-ogy was realized by the following conditions:ethanol concentration was 70%,ethanol was multiplied with 6.5 times,and extraction time was 2 hours.The maximal yield of total dioscin reached to 0.065 8 g/g.Regression coefficient of the binomial fittingcomplex model was 0.8 475.Conclusion:The technology can be used as the extraction process of total dioscin from Helleborus thibetanus Franch of Shaanxi.