CAJ | 학술논문

目的：观察新加良附方联合5-Fu 对荷人胃癌移植瘤裸鼠血清血管内皮生长因子（Vascular Endothelial Growth Factor， VEGF）、碱性成纤维生长因子（Basic Fibroblast Growth Factor，bFGF）水平的影响。方法：建立移植性人胃癌细胞（SGC-7901）裸鼠移植瘤模型，并将模型动物随机分为空白组、5-Fu 组及新加良附方高、中、低剂量组、联合给药组（新加良附中剂量＋5-Fu 组）6组。新加良附方高、中、低剂量组给药剂量分别为10 g·d －1·kg －1、5 g·d －1·kg －1、2.5 g·d －1·kg －1；5-Fu 组给药剂量为17 mg·d －1·kg －1；空白组给予等量无菌生理盐水，新加良附方联合5-Fu 组剂量为新加良附方中剂量（5 g·d －1·kg －1）联合应用5-Fu（17 mg·d －1·kg －1）。给药结束后，采用免疫学方法（ELISA）检测裸鼠血清中 VEGF、bFGF 的含量。结果：VEGF 含量显示：联合给药组，5-Fu 组，新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低（P ＜0.01），新加良附各剂量组对VEGF 含量减低作用具有一定的剂量依赖性，联合用药组 VEGF 含量低于5-Fu 组、新加良附中剂量组，但无统计学意义（P ＞0.05）。bFGF 含量显示：联合给药组，5-Fu 组，新加良附大、中、小剂量组裸鼠血清中含量较空白对照组均明显降低（P ＜0.01）。结论：新加良附方可以下调人胃癌裸鼠的血清中 VEGF、bFGF 表达水平，提示新加良附方具有一定抗胃癌新生血管生成作用。
목적：관찰신가량부방연합5-Fu 대하인위암이식류라서혈청혈관내피생장인자（Vascular Endothelial Growth Factor， VEGF）、감성성섬유생장인자（Basic Fibroblast Growth Factor，bFGF）수평적영향。방법：건립이식성인위암세포（SGC-7901）라서이식류모형，병장모형동물수궤분위공백조、5-Fu 조급신가량부방고、중、저제량조、연합급약조（신가량부중제량＋5-Fu 조）6조。신가량부방고、중、저제량조급약제량분별위10 g·d －1·kg －1、5 g·d －1·kg －1、2.5 g·d －1·kg －1；5-Fu 조급약제량위17 mg·d －1·kg －1；공백조급여등량무균생리염수，신가량부방연합5-Fu 조제량위신가량부방중제량（5 g·d －1·kg －1）연합응용5-Fu（17 mg·d －1·kg －1）。급약결속후，채용면역학방법（ELISA）검측라서혈청중 VEGF、bFGF 적함량。결과：VEGF 함량현시：연합급약조，5-Fu 조，신가량부대、중、소제량조라서혈청중함량교공백대조조균명현강저（P ＜0.01），신가량부각제량조대VEGF 함량감저작용구유일정적제량의뢰성，연합용약조 VEGF 함량저우5-Fu 조、신가량부중제량조，단무통계학의의（P ＞0.05）。bFGF 함량현시：연합급약조，5-Fu 조，신가량부대、중、소제량조라서혈청중함량교공백대조조균명현강저（P ＜0.01）。결론：신가량부방가이하조인위암라서적혈청중 VEGF、bFGF 표체수평，제시신가량부방구유일정항위암신생혈관생성작용。
Objective:To observe the effect of Modified Liang-fu Formula (MLFF)combined with 5-Fu on VEGF and bFGF expression in the serum of human gastric cancer xenografted nude mice.Methods:The animal model of SGC-7901 xenografted nude mice was estab-lished,and all the mice were randomly divided into 6 groups;namely model control group (the same amount of normal sterile saline),5-Fu group (17 mg·d -1 ·kg -1 ),MLFF high-dose group (10 g·d -1 ·kg -1 ),MLFF medium-dose group (5 g·d -1 ·kg -1 ),MLFF low-dose group (2.5 g·d -1 ·kg -1 )and drug combination group (5 g·d -1 ·kg -1 MLFF combined with 17 mg·d -1 ·kg -1 5-Fu). After 10 days of continuous treatment,eyeball blood was withdrew,the mice were killed,and the expression of VEGF and bFGF in the se-rum were detected using ELISA method.Results:Compared to model control group,the expression levels of VEGF in nude mice serum were significantly reduced in 5-Fu group,MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively (P <0.01),while MLFF reduced VEGF expression in a dose-dependent manner.The combination group showed a lower level of VEGF compared to 5-Fu group and MLFF medium-dose group,but there was no significant difference (P <0.05).Mean-while,compared to model control group,the expression level of bFGF in nude mice serum was significantly decreased in 5-Fu group, MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively (P <0.01).Conclu-sion:MLFF can down-regulate the expression of VEGF,bFGF in the serum of human gastric cancer xenografted nude mice.It indicates that MLFF has the potential to serve as anti-angiogenesis agent for gastric cancer.