世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2014年
12期
1612-1615
,共4页
迁延性腹泻%自拟止泻方%Th17 细胞%IL-6%IL-17%IL-23
遷延性腹瀉%自擬止瀉方%Th17 細胞%IL-6%IL-17%IL-23
천연성복사%자의지사방%Th17 세포%IL-6%IL-17%IL-23
Protracted diarrhea%Self-made antidiarrheal decoction%Th17 cells%IL-6%IL-17%IL-23
目的：观察自拟止泻方对小儿迁延性腹泻的临床疗效以及其部分调节机制的研究。方法：将本院2010年3月至2013年3月100例延性腹泻的患儿纳入研究，对照组50例患儿接受内科常规治疗，观察组50例在对照组治疗基础上加用自拟止泻方，观察2组患儿均以10 d 为1个疗程，疗程结束后观察2组患儿临床症状的变化，同时采用流式细胞术检测外周血中 Th17细胞表达水平，ELISA 检测患者血清中 IL-6、IL-17、IL-23的蛋白表达水平。结果：1）2组患儿体温恢复时间、呕吐停止时间比较差异无统计学意义（P ＞0.05），但是观察组患儿腹泻停止时间短于对照组（P ＜0.05）；2）治疗后2组患儿血清中 IL-6、IL-17、IL-23水平低于治疗前（P ＜0.05），治疗1个疗程后观察组患者 IL-17、IL-6、IL-23水平下降趋势较对照组患儿明显（P ＜0.01）；3）随访3个月后发现，观察组患儿的体重增长明显高于对照组患儿（P ＜0.05）。结论：迁延性腹泻患儿存在 Th17细胞表达增加，功能亢进，自拟止泻方可以明显下调 Th17细胞表达频数及功能，该效应可能是自拟止泻方从机体免疫方面改善迁延性腹泻的作用机制之一。
目的：觀察自擬止瀉方對小兒遷延性腹瀉的臨床療效以及其部分調節機製的研究。方法：將本院2010年3月至2013年3月100例延性腹瀉的患兒納入研究，對照組50例患兒接受內科常規治療，觀察組50例在對照組治療基礎上加用自擬止瀉方，觀察2組患兒均以10 d 為1箇療程，療程結束後觀察2組患兒臨床癥狀的變化，同時採用流式細胞術檢測外週血中 Th17細胞錶達水平，ELISA 檢測患者血清中 IL-6、IL-17、IL-23的蛋白錶達水平。結果：1）2組患兒體溫恢複時間、嘔吐停止時間比較差異無統計學意義（P ＞0.05），但是觀察組患兒腹瀉停止時間短于對照組（P ＜0.05）；2）治療後2組患兒血清中 IL-6、IL-17、IL-23水平低于治療前（P ＜0.05），治療1箇療程後觀察組患者 IL-17、IL-6、IL-23水平下降趨勢較對照組患兒明顯（P ＜0.01）；3）隨訪3箇月後髮現，觀察組患兒的體重增長明顯高于對照組患兒（P ＜0.05）。結論：遷延性腹瀉患兒存在 Th17細胞錶達增加，功能亢進，自擬止瀉方可以明顯下調 Th17細胞錶達頻數及功能，該效應可能是自擬止瀉方從機體免疫方麵改善遷延性腹瀉的作用機製之一。
목적：관찰자의지사방대소인천연성복사적림상료효이급기부분조절궤제적연구。방법：장본원2010년3월지2013년3월100례연성복사적환인납입연구，대조조50례환인접수내과상규치료，관찰조50례재대조조치료기출상가용자의지사방，관찰2조환인균이10 d 위1개료정，료정결속후관찰2조환인림상증상적변화，동시채용류식세포술검측외주혈중 Th17세포표체수평，ELISA 검측환자혈청중 IL-6、IL-17、IL-23적단백표체수평。결과：1）2조환인체온회복시간、구토정지시간비교차이무통계학의의（P ＞0.05），단시관찰조환인복사정지시간단우대조조（P ＜0.05）；2）치료후2조환인혈청중 IL-6、IL-17、IL-23수평저우치료전（P ＜0.05），치료1개료정후관찰조환자 IL-17、IL-6、IL-23수평하강추세교대조조환인명현（P ＜0.01）；3）수방3개월후발현，관찰조환인적체중증장명현고우대조조환인（P ＜0.05）。결론：천연성복사환인존재 Th17세포표체증가，공능항진，자의지사방가이명현하조 Th17세포표체빈수급공능，해효응가능시자의지사방종궤체면역방면개선천연성복사적작용궤제지일。
Objective:To observe the clinical curative effect of self-made antidiarrheal decoction in the treatment of pediatric protracted diarrhea and some of its regulatory mechanism.Methods:One hundred cases of pediatric protracted diarrhea treated in our hospital dur-ing March 2010 to March 2013 were included in the study.Fifty cases in the control group accepted medical conventional treatment,and the other 50 cases in the observation group received self-made antidiarrheal decoction apart from the same treatment of the control group. The treatment course of both groups was 10 days.The symptoms changes of children in the two groups after treatment was observed.Sim-ultaneously flow cytometry was used to test peripheral blood expression level of Th17 cells,and ELISA was used to detect the protein ex-pression level of IL-6,IL-17,IL-23 in the serum of patients.Results:1)Temperature recovery time,vomiting stop time of the two groups were with no statistical differences (P >0.05),but the diarrhea stop time of the observation group was shorter than that of the control group (P <0.05).2)after treatment,serum IL-6,IL-17,IL-23 levels in children in the two groups with lower than those be-fore treatment (P <0.05),and after one period of treatment,IL-17,IL-6,IL-23 levels of the observation group declined significantly than those of the control group (P <0.01).3)after the 3 months follow-up,childrens weight gain of the observation group was obvi-ously more than that of the control group (P <0.05).Conclusion:Children with protracted diarrhea have increased expression of Thl7 cells and hyperfunction,and self-made antidiarrheal decoction can obviously down-regulate frequency and function of Thl7 cells expres-sion which may be one of the mechanisms of self-made antidiarrheal decoction improving protracted diarrhea in the aspects of the bodys immune ability.
