贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
12期
161-164
,共4页
生防细菌 M2%青枯劳尔氏菌%毒力测定%生防效果
生防細菌 M2%青枯勞爾氏菌%毒力測定%生防效果
생방세균 M2%청고로이씨균%독력측정%생방효과
biocontrol bacterium M2%Ralstonia solanacearum%toxicity test%biocontrol effect
为明确生防细菌M2对茄青枯劳尔氏菌的抑制作用，采用细菌 OD值法和抑菌圈法分别测定发酵液对茄青枯劳尔氏菌的抑制作用和发酵液的效价，并在温室试验其生防效果。结果表明：生防细菌 M2对茄青枯劳尔氏菌有较好的抑制活性，其 EC50为1．83×108 cfu／mL。在浓度为1×108 cfu／mL 时对病原菌的抑制率为69．05％。根据抑菌圈半径平方与浓度对数的线性关系，比较生防细菌相对硫酸链霉素的效价，108 cfu／mL 生防细菌 M2相对理论效价为浓度211．02 mg／L 的硫酸链霉素。生防细菌 M2的温室试验生防效果为56．2％。
為明確生防細菌M2對茄青枯勞爾氏菌的抑製作用，採用細菌 OD值法和抑菌圈法分彆測定髮酵液對茄青枯勞爾氏菌的抑製作用和髮酵液的效價，併在溫室試驗其生防效果。結果錶明：生防細菌 M2對茄青枯勞爾氏菌有較好的抑製活性，其 EC50為1．83×108 cfu／mL。在濃度為1×108 cfu／mL 時對病原菌的抑製率為69．05％。根據抑菌圈半徑平方與濃度對數的線性關繫，比較生防細菌相對硫痠鏈黴素的效價，108 cfu／mL 生防細菌 M2相對理論效價為濃度211．02 mg／L 的硫痠鏈黴素。生防細菌 M2的溫室試驗生防效果為56．2％。
위명학생방세균M2대가청고로이씨균적억제작용，채용세균 OD치법화억균권법분별측정발효액대가청고로이씨균적억제작용화발효액적효개，병재온실시험기생방효과。결과표명：생방세균 M2대가청고로이씨균유교호적억제활성，기 EC50위1．83×108 cfu／mL。재농도위1×108 cfu／mL 시대병원균적억제솔위69．05％。근거억균권반경평방여농도대수적선성관계，비교생방세균상대류산련매소적효개，108 cfu／mL 생방세균 M2상대이론효개위농도211．02 mg／L 적류산련매소。생방세균 M2적온실시험생방효과위56．2％。
The inhibition effect and titer of biocontrol bacterium M2 fermentation liquor against R.solanacearum were separately determined by bacterial OD value and inhibition zone method,and the biocontrol efficiency was tested in the greenhouse to definite the inhibition effect of biocontrol bacterium M2 against Ralstonia solanacearum.The results showed that biocontrol bacterium M2 has the good inhibition effect against R.solanacearum and its EC50 is 1.83×108 cfu/mL The inhibition rate of biocontrol bacterium M2 with 1 × 108 cfu/m concentration against R.solanacearum reaches 69.05%.The titer of biocontrol bacterium M2 and corresponding Streptomycin sulfate is compared according to the linear relation between inhibition zone radius square and concentration logarithm.The relative theoretical titer of biocontrol bacterium M2 with 108 cfu/mL is equal to Streptomycin sulfate with 211.02 mg/L concentration.The biocontrol efficiency of biocontrol bacterium M2 is up to 56.2% in the greenhouse.
