CAJ | 학술논문

维生素D3本身没有生物活性，在体内需经羟化生成活性维生素 D3才能发挥其生理作用。目前主要通过化学合成法和微生物转化法来生产活性维生素 D3。化学合成过程复杂、污染严重，不适合工业化生产。而生物转化法条件温和，特异性强，反应步骤少，在制备活性维生素 D3中具有重要意义。综述了参与维生素 D3代谢的哺乳动物 CYP450羟化酶、维生素 D3羟化菌株及生产过程中采用的基因工程方法，并展望研究方向的发展趋势。
유생소D3본신몰유생물활성，재체내수경간화생성활성유생소 D3재능발휘기생리작용。목전주요통과화학합성법화미생물전화법래생산활성유생소 D3。화학합성과정복잡、오염엄중，불괄합공업화생산。이생물전화법조건온화，특이성강，반응보취소，재제비활성유생소 D3중구유중요의의。종술료삼여유생소 D3대사적포유동물 CYP450간화매、유생소 D3간화균주급생산과정중채용적기인공정방법，병전망연구방향적발전추세。
Vitamin D3 is biologically inactive and needs to be sequentially hydroxylated before acquiring its physiological activity.Now,there are two ways,chemical syn thesis and biological transformation,to produce active vitamin D3. The former synthesis requires a complex procedure and pollutes the environment,making it unavailable for industrialized production.In contrast to the chemical synthesis, biotransformation is a single-step process,which is mostly preferred because of its special selectivities and the mild conditions.Consequently,biological transformation of preparing active vitamin D3 is extremely significant.This authors summarized the mammalian P450 enzymes and the microorganisms involved in VD3 metabolism,meanwhile,the genetic engineering which is used is instructed and the future studies are prospected.