CAJ | 학술논문

为探明三尖杉内生真菌S19的次生代谢产物的结构与活性，采用形态学特征和ITS序列鉴定菌株，利用硅胶柱层析、Sephadex LH-20柱层析和重结晶等方法对该菌株的发酵产物进行分离、纯化，并结合所得化合物的理化性质和波谱数据鉴定其结构，通过测试菌对其次生代谢产物进行活性测试。结果表明：菌株 S19为黄曲霉（Aspergillus flavus ），从其发酵产物中分离得到6个化合物，分别为5-羟甲基呋喃-3-羧酸（1）、麦角甾醇（2）、β-谷甾醇（3）、豆甾-7，22-二烯-3β，5α，6α-三醇（4）、胶霉毒素（5）和丁二酸（6）。黄曲霉（S19）可以大量产生5-羟甲基呋喃-3-羧酸（1）。化合物1对大肠杆菌抑菌活性较好，其 MIC 值为15．6μg／mL；化合物5对测试菌均有很好的抑菌活性。
위탐명삼첨삼내생진균S19적차생대사산물적결구여활성，채용형태학특정화ITS서렬감정균주，이용규효주층석、Sephadex LH-20주층석화중결정등방법대해균주적발효산물진행분리、순화，병결합소득화합물적이화성질화파보수거감정기결구，통과측시균대기차생대사산물진행활성측시。결과표명：균주 S19위황곡매（Aspergillus flavus ），종기발효산물중분리득도6개화합물，분별위5-간갑기부남-3-최산（1）、맥각치순（2）、β-곡치순（3）、두치-7，22-이희-3β，5α，6α-삼순（4）、효매독소（5）화정이산（6）。황곡매（S19）가이대양산생5-간갑기부남-3-최산（1）。화합물1대대장간균억균활성교호，기 MIC 치위15．6μg／mL；화합물5대측시균균유흔호적억균활성。
The fermentation product of endophytic fungus S1 9 strain in C .fortunei identified by morphological feature and ITS sequence was isolated and purified by SGCCT,Sephadex LH-20 column chromatography and recrystallization methods,then their structures were identified according to their physicochemical property and spectrum data, and finally the activity of secondary metabolites was determined by testing bacteria.The results showed that S19 strain is identified as Aspergillus flavus ,and six compounds of 5-hydroxymethyl furfuran-3-carboxylic acid,ergosterol,β-sitosterol,stigmasta-7,22-diene-3β,5α,6α-triol, gliotoxin and succinic acid are isolated from its fermentation product.5-hydroxymethyl furfuran-3-carboxylic acid has good bacteriostatic activity with MIC value of 15.6 μg/mL against Escherichia coli . Gliotoxin is of good bacteriostatic activity against four testing bacteria of Pseudomonas aeruginosa ,Escherichia coli ,Staphylococcus aureus and Streptococcus lactis .