贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
12期
115-117,121
,共4页
蒲金基%杨石有%刘晓妹%胡强%张贺
蒲金基%楊石有%劉曉妹%鬍彊%張賀
포금기%양석유%류효매%호강%장하
芒果炭疽病菌%代森锰锌%敏感性基线%抗药性
芒果炭疽病菌%代森錳鋅%敏感性基線%抗藥性
망과탄저병균%대삼맹자%민감성기선%항약성
Colletotrichum gloeosporioiles%mancozeb%sensibility baseline%drug resistance
为了解芒果炭疽病菌对代森锰锌的敏感性,在多年未使用农药的野生芒果种植区采集获得64个单孢菌株,通过生长速率法测定其对代森锰锌敏感性,EC50平均值为(19.59±17.71)μg/mL,以此作为敏感性基线评价采集自国内及泰国、美国等芒果产区128株芒果炭疽病菌对代森锰锌的敏感性。不同菌株对代森锰锌的敏感性有明显的差异,EC50值为0.09~3232.09μg/mL,均值为(284.64±445.28)μg/mL。不同地区菌株对代森锰锌的敏感性不同,菌株抗性频率以云南的最高,广西其次,美国的最低,其他地区的以低抗为主,广西和广东均出现了高抗菌株。不同部位菌株分离自叶片的最多,茎的最少,叶和果均出现了高抗菌株。
為瞭解芒果炭疽病菌對代森錳鋅的敏感性,在多年未使用農藥的野生芒果種植區採集穫得64箇單孢菌株,通過生長速率法測定其對代森錳鋅敏感性,EC50平均值為(19.59±17.71)μg/mL,以此作為敏感性基線評價採集自國內及泰國、美國等芒果產區128株芒果炭疽病菌對代森錳鋅的敏感性。不同菌株對代森錳鋅的敏感性有明顯的差異,EC50值為0.09~3232.09μg/mL,均值為(284.64±445.28)μg/mL。不同地區菌株對代森錳鋅的敏感性不同,菌株抗性頻率以雲南的最高,廣西其次,美國的最低,其他地區的以低抗為主,廣西和廣東均齣現瞭高抗菌株。不同部位菌株分離自葉片的最多,莖的最少,葉和果均齣現瞭高抗菌株。
위료해망과탄저병균대대삼맹자적민감성,재다년미사용농약적야생망과충식구채집획득64개단포균주,통과생장속솔법측정기대대삼맹자민감성,EC50평균치위(19.59±17.71)μg/mL,이차작위민감성기선평개채집자국내급태국、미국등망과산구128주망과탄저병균대대삼맹자적민감성。불동균주대대삼맹자적민감성유명현적차이,EC50치위0.09~3232.09μg/mL,균치위(284.64±445.28)μg/mL。불동지구균주대대삼맹자적민감성불동,균주항성빈솔이운남적최고,엄서기차,미국적최저,기타지구적이저항위주,엄서화엄동균출현료고항균주。불동부위균주분리자협편적최다,경적최소,협화과균출현료고항균주。
The sensibility of 128 C.gloeosporioiles strains from different provinces in China,Thailand and America against mancozeb was evaluated by the sensibility basic line (19.59 17.71 μg/mL of mean EC50 value )by determining the sensitivity of 64 strains collected from wild mango cultivation areas without applying pesticides for many years against mancozeb to study the sensibility of C.gloeosporioiles against mancozeb.There are obvious differences in the sensibility among different strains and their EC50 value range is 0.09~3 232.09 μg/mL with 284.64±445.28 μg/mL of the mean EC50 value.The highest drug resistance is the strains from Yunnan,followed by the strains from Guangxi,and the lowest drug resistance is the strains from America.The strains with a higher resistance are from Guangxi and Guangdong but the strains with a lower resistance are from other areas.The most strains are isolated from mango leaves and the strains with a higher resistance can be isolated from leaves and fruits of mango either.