CAJ | 학술논문

为明确威宁中华蟾蜍的分子分类学地位，对采自贵州省威宁县的蟾蜍标本（标本号：LPSWN01A）进行线粒体12S rRNA 基因扩增和双重单系法分析。结果表明：该标本的序列大小为906 bp （GenBank 登录号：KJ803016），基于数据集 A 构建的系统发育树中，威宁中华蟾蜍与7条中华蟾蜍同源序列聚为一个单系（中华蟾蜍支系），威宁中华蟾蜍以较低的最大似然率独立为一个支系；基于数据集 B 构建的系统发育树中，威宁中华蟾蜍与2个中华蟾蜍华西亚种种群聚为一支，因此推断威宁产蟾蜍属于中华蟾蜍华西亚种（Bufo gararizans andrewsi ）。另外，威宁中华蟾蜍与云南怒江种群的遗传距离最近，为0．0093，说明威宁中华蟾蜍属于中华蟾蜍华西亚种，与之前的传统分类文献记载一致。
위명학위저중화섬서적분자분류학지위，대채자귀주성위저현적섬서표본（표본호：LPSWN01A）진행선립체12S rRNA 기인확증화쌍중단계법분석。결과표명：해표본적서렬대소위906 bp （GenBank 등록호：KJ803016），기우수거집 A 구건적계통발육수중，위저중화섬서여7조중화섬서동원서렬취위일개단계（중화섬서지계），위저중화섬서이교저적최대사연솔독립위일개지계；기우수거집 B 구건적계통발육수중，위저중화섬서여2개중화섬서화서아충충군취위일지，인차추단위저산섬서속우중화섬서화서아충（Bufo gararizans andrewsi ）。령외，위저중화섬서여운남노강충군적유전거리최근，위0．0093，설명위저중화섬서속우중화섬서화서아충，여지전적전통분류문헌기재일치。
The mitochondria 12S rRNA gene of Weining B.gargarizans sample (LPSWN01A)was amplified and analyzed by dual monophyletic method to definite molecular taxonomic status of B. gargarizans.The sequence length of Weining B.gargarizans is 906bp (GenBank accession number:KJ803016).The sequence of Weining B.gargarizans and seven homologous sequences of B.gargarizans form a monosystem in the phyogenetic tree built by data set A. Weining B.gargarizans forms an independent clade with lower maximum likelihood. Weining B.gargarizans and two B.gargarizans andrewsi populations form a monosystem in the phyogenetic tree built by data set B,which implies that Weining B.gargarizans belongs to B.gargarizans andrewsi .In addition,the genetic distance between Weining B.gargarizans and Nujiang River population is 0.009 3 only,which proves that Weining B. gargarizans belongs to B.gargarizans andrewsi also.In conclusion,the molecular identification result of Weining B.gargarizans is in accordance with the documental records of previous classical taxonomy.