贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
12期
23-26
,共4页
糯玉米%自交系%SSR%遗传多样性
糯玉米%自交繫%SSR%遺傳多樣性
나옥미%자교계%SSR%유전다양성
waxy corn%inbred line%SSR%genetic diversity
为筛选出形态学表现好、遗传距离较远的糯玉米自交系类群,利用 SSR分子标记技术对表型不同的101份糯玉米自交系进行 SSR 分子标记遗传多样性分析。结果表明,30对 SSR 引物筛选出23对多态性好、带型稳定的引物,分布在玉米所有染色体上;共检测出128个条带,108个等位基因,平均每个引物检测出4.7个等位基因;23对引物多态性信息量为0.59~0.94,平均为0.85;SSR 聚类结果将101份糯玉米自交系分为10大类群。
為篩選齣形態學錶現好、遺傳距離較遠的糯玉米自交繫類群,利用 SSR分子標記技術對錶型不同的101份糯玉米自交繫進行 SSR 分子標記遺傳多樣性分析。結果錶明,30對 SSR 引物篩選齣23對多態性好、帶型穩定的引物,分佈在玉米所有染色體上;共檢測齣128箇條帶,108箇等位基因,平均每箇引物檢測齣4.7箇等位基因;23對引物多態性信息量為0.59~0.94,平均為0.85;SSR 聚類結果將101份糯玉米自交繫分為10大類群。
위사선출형태학표현호、유전거리교원적나옥미자교계류군,이용 SSR분자표기기술대표형불동적101빈나옥미자교계진행 SSR 분자표기유전다양성분석。결과표명,30대 SSR 인물사선출23대다태성호、대형은정적인물,분포재옥미소유염색체상;공검측출128개조대,108개등위기인,평균매개인물검측출4.7개등위기인;23대인물다태성신식량위0.59~0.94,평균위0.85;SSR 취류결과장101빈나옥미자교계분위10대류군。
The genetic diversity of 1 0 1 waxy corn inbred lines were analyzed by SSR molecular marker technology to screen waxy corn inbred lines with good morphology performance and further genetic distance.Results:23 pairs of stable type and good polymorphic primers were screened out from 30 pairs of SSR primers,distributing on all maize chromosomes,amplifying 128 bands and 108 alleles were detected, 4.7 alleles on average per primer.The value of polymorphism information content (PIC)for 23 pairs of primers varied from 0.59 to 0.94,with an average of 0.85.The results of clustering analysis showed that the 101 inbred lines were divided into 10 groups.