CAJ | 학술논문

为了解重庆市玉米自交系的遗传多样性，提高育种效率，利用32对 SSR引物对66份玉米自交系进行检测，分析其遗传相似系数，并进行聚类分析，掌握材料间的亲缘关系。结果表明：1）共检测出307个条带，每对引物检测到条带4～25个，平均9．59个，其中多态性条带占80．78％；多态性信息量（PIC 值）变幅为0．516～0．988，平均为0．721；供试自交系间遗传相似系数变化范围为0．54～0．88，平均0．68，遗传相似系数在0．60～0．70间分布最多，占60．33％，供试材料亲缘关系较近，但也有一定的遗传差异。2）聚类分析将供试材料分为6大类群，其中，改良 Reid 群和旅大红骨类群分别占24．24％和60．60％。
위료해중경시옥미자교계적유전다양성，제고육충효솔，이용32대 SSR인물대66빈옥미자교계진행검측，분석기유전상사계수，병진행취류분석，장악재료간적친연관계。결과표명：1）공검측출307개조대，매대인물검측도조대4～25개，평균9．59개，기중다태성조대점80．78％；다태성신식량（PIC 치）변폭위0．516～0．988，평균위0．721；공시자교계간유전상사계수변화범위위0．54～0．88，평균0．68，유전상사계수재0．60～0．70간분포최다，점60．33％，공시재료친연관계교근，단야유일정적유전차이。2）취류분석장공시재료분위6대류군，기중，개량 Reid 군화려대홍골류군분별점24．24％화60．60％。
The genetic similar coefficient of 66 maize inbred lines was determined by 32 pairs of SSR primers and their genetic relationship was analyzed by clustering analysis to study the genetic diversity of maize inbred lines and improve breeding efficiency in Chongqing.The results showed that:1)307 bands are detected by 32 pairs of primers and 4-25 bands can be detected by a pairs of primers. The polymorphism band accounts for 80.78% and the variation range of PIC is 0.516~0.988 with an average of 0.721.The variation range of genetic similar coefficient among tested inbred lines is 0.54~0.88 with an average of 0.68.Most genetic similar coefficient is 0.60~0.70,accounts for 60.33%,which indicates that the genetic relationship among tested inbred lines is closer but there still is a certain difference in genetic relationship;2)66 inbred lines can be divided into 6 groups.The modified Reid group and Ludahonggu group accounts for 24.24% and 60.60% respectively. Other germplasm groups, especially tropical germplasm resources should be used in maize breeding in Chongqing.