CAJ | 학술논문

为克服传统繁殖方法繁殖系数低和周期长等缺点，实现龙芽草的快速繁殖，以龙芽草（Agri-monia pilosa ）叶片为外植体和 MS 为基本培养基，利用不同生长调节物质及其组合，研究建立了龙芽草组织培养的快速繁殖体系。结果表明：细胞分裂素可直接诱导叶片产生不定芽，MS＋TDZ 0．3 mg／L＋6-BA 2．0 mg／L为适宜培养基；增殖生长需添加生长素，适宜的培养基为 MS ＋ TDZ 0．3 mg／L ＋6-BA 2．0 mg／L＋IAA 1．0 mg／L；肌醇不影响生根，适宜生根的培养基为1／4 MS＋NAA 0．1 mg／L。
위극복전통번식방법번식계수저화주기장등결점，실현룡아초적쾌속번식，이룡아초（Agri-monia pilosa ）협편위외식체화 MS 위기본배양기，이용불동생장조절물질급기조합，연구건립료룡아초조직배양적쾌속번식체계。결과표명：세포분렬소가직접유도협편산생불정아，MS＋TDZ 0．3 mg／L＋6-BA 2．0 mg／L위괄의배양기；증식생장수첨가생장소，괄의적배양기위 MS ＋ TDZ 0．3 mg／L ＋6-BA 2．0 mg／L＋IAA 1．0 mg／L；기순불영향생근，괄의생근적배양기위1／4 MS＋NAA 0．1 mg／L。
To overcome the shortcomings of low propagation coefficients and long cyclicality,and realize the rapid propagation of A.pilosa,leaf and MS were use as explants and basic medium separately to construct rapid propagation system by using different growth regulatory substances and their combinations.The results showed cytokinin could induce adventitious buds.The optimal medium for adventitious buds was MS+TDZ 0.3 mg/L+6-BA 2.0 mg/L;Auxin was needed for proliferation culture, the optimal medium for proliferation was MS + TDZ 0.3 mg/L + 6-BA 2.0 mg/L + IAA 1.0 mg/L;Rooting culture test showed that inositol had no effect on rooting.So the optimal medium for rooting culture was 1/4MS+NAA 0.1 mg/L.