CAJ | 학술논문

为获得含量较高的五味子多糖，采用响应面优化试验法、正交试验法分别对五味子多糖的提取、脱色及脱蛋白工艺进行优化。结果表明：五味子多糖的最佳提取工艺条件为药材粉碎过筛60目，料液比1∶30（g／mL），超声功率165．31W，萃取38．75 min；活性炭脱色最佳工艺为调节 pH 5．0，脱色温度80℃，活性炭用量1．5％，搅拌30 min；脱蛋白的最佳工艺条件为加入 Sevag 试剂（氯仿∶正丁醇＝4∶1），每次振荡20 min，样液与试剂比为5∶1，重复处理5次。在此优化条件下，五味子多糖提取率达10．52％，纯度由41．76％增加到79．53％。
위획득함량교고적오미자다당，채용향응면우화시험법、정교시험법분별대오미자다당적제취、탈색급탈단백공예진행우화。결과표명：오미자다당적최가제취공예조건위약재분쇄과사60목，료액비1∶30（g／mL），초성공솔165．31W，췌취38．75 min；활성탄탈색최가공예위조절 pH 5．0，탈색온도80℃，활성탄용량1．5％，교반30 min；탈단백적최가공예조건위가입 Sevag 시제（록방∶정정순＝4∶1），매차진탕20 min，양액여시제비위5∶1，중복처리5차。재차우화조건하，오미자다당제취솔체10．52％，순도유41．76％증가도79．53％。
The extraction and purification technology conditions of polysaccharides from S .chinensis was optimized to get the highest content of polysaccharides.Response surface methodology and orthogonal test were employed to optimize the extraction,decoloration and deproteinization conditions,respectively. Results:The optimum extraction conditions with ultrasound-assisted method were particle size of sample sieved with 60 mesh,ultrasound power of 165.31 W,solid to liquid ratio of 1∶30 and extraction time of 38.75 min;The optimum decoloration conditions were pH of 5.0,temperature of 80℃,amount of active carbon of 1.5%,shaking time of 30 min;and the optimum deproteinization conditions were Sevag reagent (chloroform/normal butanol=4∶1),shaking time of 20 min,sample liquid to Sevag regent ratio of 1∶5 and deproteinization times of 5 times.Under these optimized conditions,the extraction efficiency of polysaccharides reached 10.52%,and the purity of polysaccharides increased from 41.67% to 79.53%.