CAJ | 학술논문

目的检测急性心肌梗死早期（发病到就诊时间＜2h）患者血清中的蛋白表达谱，筛选并鉴定出特异的标志蛋白。方法选取20例急性心肌梗死早期患者（早期急性心肌梗死组）和40例稳定性心绞痛患者（稳定性心绞痛组），采用激光解析电离飞行时间质谱技术，检测两组患者血清中的蛋白峰强度，寻找差异表达蛋白并建立蛋白分类模式，确定诊断的特异度和敏感度。采用液相质谱技术进一步鉴定已筛选出来的蛋白。结果早期急性心肌梗死组有5个蛋白显著高表达，质荷比分别为1530.32、4301.46、8683.44、23386.1、23625.5Da，与稳定性心绞痛组患者比较，差异有统计学意义（P＜0.01）。建立决策树，早期诊断急性心肌梗死的敏感度为93.33﹪，特异度为85.00%，阳性预测值为93.33%。经过鉴定，phosphoglycerate mutase 1在急性心肌梗死早期患者中高表达。结论通过蛋白质组学技术可以早期发现急性心肌梗死，phosphoglycerate mutase 1为早期特异表达蛋白。
목적검측급성심기경사조기（발병도취진시간＜2h）환자혈청중적단백표체보，사선병감정출특이적표지단백。방법선취20례급성심기경사조기환자（조기급성심기경사조）화40례은정성심교통환자（은정성심교통조），채용격광해석전리비행시간질보기술，검측량조환자혈청중적단백봉강도，심조차이표체단백병건립단백분류모식，학정진단적특이도화민감도。채용액상질보기술진일보감정이사선출래적단백。결과조기급성심기경사조유5개단백현저고표체，질하비분별위1530.32、4301.46、8683.44、23386.1、23625.5Da，여은정성심교통조환자비교，차이유통계학의의（P＜0.01）。건립결책수，조기진단급성심기경사적민감도위93.33﹪，특이도위85.00%，양성예측치위93.33%。경과감정，phosphoglycerate mutase 1재급성심기경사조기환자중고표체。결론통과단백질조학기술가이조기발현급성심기경사，phosphoglycerate mutase 1위조기특이표체단백。
Objective To screen early serum protein markers for acute myocardial infarction by SELDI- TOF mass spec-trometry. Methods Twenty patients with acute myocardial infarction admitted within 2h after onset of symptoms, and 40 patients with stable angina pectoris were enrol ed in this study. Serum protein profile was analyzed by surface enhanced laser desorption ionization- time of flight- mass spectrometry (SELDI- TOF- MS). The differential y expressed proteins were identified by high per-formance 1iquid chromatography- electrospray ionization mass spectrometry (LC- MS). Results Compared to stable angina pectoris, 5 proteins were significantly over- expressed in acute myocardial infarction(P<0.01): 1 530.32, 4 301.46, 8 683.44, 23 386.1, 23 625.5Da. Based on differential y expressed proteins a diagnosis model for acute myocardial infarction was con-structed. The sensitivity, specificity and positive predictive value of this model were 93.33%, 85%and 93.33%, respectively. A-mong 5 differential y expressed proteins phosphoglycerate mutase 1 was identified, which was over- expressed in patients with early stage myocardial infarction. Conclusion Some serum proteins are over- expressed in patients with acute myocardial in-farction including phosphoglycerate mutase 1.