CAJ | 학술논문

目的检测胃腺癌、印戒细胞癌组织及正常胃黏膜组织中8-羟基脱氧鸟苷（8- oxodGsn）水平，探讨DNA氧化损伤与不同类型胃癌临床指标间的关系。方法提取42例胃癌（腺癌28例，印戒细胞癌14例）及癌旁5cm以外的正常组织的DNA，并采用核酸酶P1和碱性磷酸酶消化成单个核苷，再采用高效液相色谱-串联质谱法（LC- MS/MS）检测其8- oxodGsn的水平；同时联合免疫组化法对组织中的8- oxodGsn进行定位分析。结果不同类型胃癌组织与正常组织中8- oxodGsn水平的差异均有统计学意义（均P＜0.05）。8- oxodGsn主要分布于肿瘤细胞的细胞质和细胞核内，腺癌细胞中以细胞核分布居多，印戒细胞癌中以细胞质分布居多。不同类型胃癌组织中8- oxodGsn水平在癌组织浸润深度、淋巴结转移程度、远处器官转移程度、临床病理分期等差异均有统计学意义（均P＜0.05）。结论8- oxodGsn水平在两种类型的胃癌组织中均有不同程度的增加，提示DNA氧化损伤可能是胃癌发生、发展的重要因素。
목적검측위선암、인계세포암조직급정상위점막조직중8-간기탈양조감（8- oxodGsn）수평，탐토DNA양화손상여불동류형위암림상지표간적관계。방법제취42례위암（선암28례，인계세포암14례）급암방5cm이외적정상조직적DNA，병채용핵산매P1화감성린산매소화성단개핵감，재채용고효액상색보-천련질보법（LC- MS/MS）검측기8- oxodGsn적수평；동시연합면역조화법대조직중적8- oxodGsn진행정위분석。결과불동류형위암조직여정상조직중8- oxodGsn수평적차이균유통계학의의（균P＜0.05）。8- oxodGsn주요분포우종류세포적세포질화세포핵내，선암세포중이세포핵분포거다，인계세포암중이세포질분포거다。불동류형위암조직중8- oxodGsn수평재암조직침윤심도、림파결전이정도、원처기관전이정도、림상병리분기등차이균유통계학의의（균P＜0.05）。결론8- oxodGsn수평재량충류형적위암조직중균유불동정도적증가，제시DNA양화손상가능시위암발생、발전적중요인소。
Objective To investigate oxidative DNA damage in gastric adenocarcinoma, signet ring cell carcinoma and normal gastric mucosa by detecting 8- dihydro- 2'- deoxyguanosine (8- oxodGsn) levels. Methods Specimens of gastric cancer tissues and normal gastric mucosa were obtained from 28 cases of adenocarcinoma and 14 cases of signet ring cel car-cinoma. The DNA was extracted from tissue specimens and digested by nuclease P1 and alkaline phosphatase. The levels of 8- oxodGsn were determined by LC- MS/MS method and the expression of 8- oxodGsn in tissue was detected by immunohisto-chemical method. Results There was a significant difference in 8- oxodGsn levels between gastric cancer and normal gastric mucosa(P<0.05). Immunohistochemical staining showed that 8- oxodGsn was mainly distributed in nucleus in gastric adenocar-cinoma, while mainly in cytoplasm in signet ring cel carcinoma. The expression levels of 8- oxodGsn were significantly correlated with invasion depth, lymph node metastasis, distant metastasis and clinical staging of gastric cancer (al P<0.05). Conclusion 8- oxodGsn levels are increased in gastric adenocarcinoma and gastric signet ring cel carcinoma in different extend, which sug-gested that oxidative DNA damage may be an important factor in the development of gastric cancer.