重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
34期
4579-4580,4583
,共3页
肾病%酸中毒,肾小管性%整合素连接激酶%梗阻性
腎病%痠中毒,腎小管性%整閤素連接激酶%梗阻性
신병%산중독,신소관성%정합소련접격매%경조성
nephrology%acidosis,renal tubular%integrin linked kinase%obstructive
目的：观察整合素连接激酶（ILK）在梗阻性肾病合并肾小管酸中毒中的表达，探讨肾小管酸中毒在致肾间质纤维化中的作用。方法实验大鼠75只，其中假手术组大鼠25只，单侧输尿管梗阻（UUO）大鼠50只。UUO大鼠于术后第7天分为肾小管酸中毒大鼠（UUO1组，n＝31）和非肾小管酸中毒大鼠（UUO2组，n＝19），同时做肾功能与组织学检测，于手术后7、14、21、28 d时分批处死大鼠，免疫组织化学与 Western blot法测定肾组织中 ILK的表达。结果与 UUO2组比较，UUO1组光镜下炎症细胞浸润和间质纤维化程度更严重；假手术组仅有少量的 ILK蛋白表达，几乎无纤粘连（FN）蛋白的沉积，UUO2组 ILK蛋白表达及 FN蛋白的沉积增多，而 UUO1组较 UUO2组增多。结论在梗阻性肾病中，肾小管酸中毒可上调 ILK蛋白的表达与FN的沉积，加速肾小管间质纤维化。
目的：觀察整閤素連接激酶（ILK）在梗阻性腎病閤併腎小管痠中毒中的錶達，探討腎小管痠中毒在緻腎間質纖維化中的作用。方法實驗大鼠75隻，其中假手術組大鼠25隻，單側輸尿管梗阻（UUO）大鼠50隻。UUO大鼠于術後第7天分為腎小管痠中毒大鼠（UUO1組，n＝31）和非腎小管痠中毒大鼠（UUO2組，n＝19），同時做腎功能與組織學檢測，于手術後7、14、21、28 d時分批處死大鼠，免疫組織化學與 Western blot法測定腎組織中 ILK的錶達。結果與 UUO2組比較，UUO1組光鏡下炎癥細胞浸潤和間質纖維化程度更嚴重；假手術組僅有少量的 ILK蛋白錶達，幾乎無纖粘連（FN）蛋白的沉積，UUO2組 ILK蛋白錶達及 FN蛋白的沉積增多，而 UUO1組較 UUO2組增多。結論在梗阻性腎病中，腎小管痠中毒可上調 ILK蛋白的錶達與FN的沉積，加速腎小管間質纖維化。
목적：관찰정합소련접격매（ILK）재경조성신병합병신소관산중독중적표체，탐토신소관산중독재치신간질섬유화중적작용。방법실험대서75지，기중가수술조대서25지，단측수뇨관경조（UUO）대서50지。UUO대서우술후제7천분위신소관산중독대서（UUO1조，n＝31）화비신소관산중독대서（UUO2조，n＝19），동시주신공능여조직학검측，우수술후7、14、21、28 d시분비처사대서，면역조직화학여 Western blot법측정신조직중 ILK적표체。결과여 UUO2조비교，UUO1조광경하염증세포침윤화간질섬유화정도경엄중；가수술조부유소량적 ILK단백표체，궤호무섬점련（FN）단백적침적，UUO2조 ILK단백표체급 FN단백적침적증다，이 UUO1조교 UUO2조증다。결론재경조성신병중，신소관산중독가상조 ILK단백적표체여FN적침적，가속신소관간질섬유화。
Objective To observe integrin linked kinase(ILK)expression in obstructive nephropathy with renal tubular acidosis, and investigate the function of renal tubular acidosis on renal interstitial fibrosis.Methods 75 rate were divided into control group and unilateral ureteral obstruction(unilateral ureteral obstruction(UUO,n=25)group,and other 50 rats in UUO group.UUO group was divided into renal tubular acidosis (UUO1 group,n=31)group and non-renal tubular acidosis(UUO2,n=19)group,re-nal function and histology test was carried at the same time.7,14,21,28 days later after operation,the rats were killed in batches, then measured the expression of ILK in renal tissue by immunohistochemistry and western blot.Results Inflammatory cell infiltra-tion and interstitial fibrosis of UUO1 group were more serious under the light microscope than that of UUO2 group;there was only a small amount of ILK protein expression in control group,almost no FN protein deposition,expression of ILK and FN protein pre-cipitation of UUO2 group increased,and UUO1 group was more than UUO2 group.Conclusion In obstructive nephropathy,renal tubular acidosis can up-regulate expression of ILK and FN sedimentation,then lead to interstitial fibrosis promotion.
