CAJ | 학술논문

目的：探讨细胞外基质金属蛋白酶诱导因子CD147在膀胱尿路上皮癌组织中的表达及其与临床病理特征和预后的关系。方法应用RT-PCR和Western blot法检测CD147 mRNA和蛋白在20例膀胱尿路上皮癌和对应的癌旁组织中的表达情况；应用组织芯片技术和免疫组织化学方法，检测CD147在108例膀胱尿路上皮癌和20例正常膀胱黏膜组织中的表达状况，分析CD147与膀胱尿路上皮癌患者的临床病理学特征及其与预后的关系。结果 CD147 mRNA和蛋白在20例膀胱尿路上皮癌组织中的表达水平明显高于相应的癌旁组织，差异有统计学意义（P＜0．01）。免疫组织化学检测发现，20例正常膀胱黏膜组织中CD147无表达或呈弱阳性表达，108例膀胱尿路上皮癌组织中CD147阳性表达率为73．1％。CD147表达与膀胱尿路上皮癌患者临床分期、病理分级、肿瘤大小和淋巴结转移密切相关（P＜0．05），而与患者的年龄、性别以及肿瘤数目无关（P＞0．05）。Kaplan-Meier生存曲线分析结果表明，高表达CD147的膀胱尿路上皮癌患者的总体生存时间明显短于 CD147低表达患者，差异有统计学意义（P＜0．01）。单因素分析结果表明，肿瘤临床分期、病理分级、淋巴结转移、肿瘤大小和CD147表达是影响膀胱尿路上皮癌患者预后的重要因素（P＜0．05）。COX多因素生存分析结果表明，肿瘤临床分期、病理分级、淋巴结转移和 CD147表达是影响膀胱尿路上皮癌患者预后的独立因素（P＜0．05）。结论 CD147在膀胱尿路上皮癌中高表达，可能与膀胱尿路上皮癌的进展有关，其表达状态是膀胱尿路上皮癌患者预后的独立因素。
목적：탐토세포외기질금속단백매유도인자CD147재방광뇨로상피암조직중적표체급기여림상병리특정화예후적관계。방법응용RT-PCR화Western blot법검측CD147 mRNA화단백재20례방광뇨로상피암화대응적암방조직중적표체정황；응용조직심편기술화면역조직화학방법，검측CD147재108례방광뇨로상피암화20례정상방광점막조직중적표체상황，분석CD147여방광뇨로상피암환자적림상병이학특정급기여예후적관계。결과 CD147 mRNA화단백재20례방광뇨로상피암조직중적표체수평명현고우상응적암방조직，차이유통계학의의（P＜0．01）。면역조직화학검측발현，20례정상방광점막조직중CD147무표체혹정약양성표체，108례방광뇨로상피암조직중CD147양성표체솔위73．1％。CD147표체여방광뇨로상피암환자림상분기、병리분급、종류대소화림파결전이밀절상관（P＜0．05），이여환자적년령、성별이급종류수목무관（P＞0．05）。Kaplan-Meier생존곡선분석결과표명，고표체CD147적방광뇨로상피암환자적총체생존시간명현단우 CD147저표체환자，차이유통계학의의（P＜0．01）。단인소분석결과표명，종류림상분기、병리분급、림파결전이、종류대소화CD147표체시영향방광뇨로상피암환자예후적중요인소（P＜0．05）。COX다인소생존분석결과표명，종류림상분기、병리분급、림파결전이화 CD147표체시영향방광뇨로상피암환자예후적독립인소（P＜0．05）。결론 CD147재방광뇨로상피암중고표체，가능여방광뇨로상피암적진전유관，기표체상태시방광뇨로상피암환자예후적독립인소。
Objective To investigate the expression of extracellular matrix metalloproteinase inducer CD147 in patients with bladder urothelial carcinoma and its relationship with clinicopathologic features and prognosis.Methods RT-PCR and Western blot were used to evaluate the mRNA and protein expression of CD147 in cancer tissues and corresponding paracanerous tissues from 20 patients with bladder urothelial carcinoma;tissue microarray containing specimens from 108 patients with bladder urothelial carcino-ma and 20 normal controls was constructed and immunohistochemistry was applied to examine the expression of CD147,and the correlations between CD147 and clinicopathological characteristics and prognosis were also analyzed.Results Expression level of CD147 mRNA and protein in cancer tissues from 20 bladder urothelial carcinoma patients was significantly higher than that of cor-responding adjacent tissues(P<0.01).Immunohistochemical detection showed normal bladder urothelium from 20 control cases were either no or weak protein expression of CD147,positive expression rate of CD147 in 108 cases of bladder urothelial carcinoma was 73.1%.the CD147 expression level had positive correlation with lymph nodes metastasis,histological grade,tumor stage and tumor size of patients with bladder urothelial carcinoma(P<0.05)and had no relationship with age,sex,the number of tumor(P>0.05).Kaplan-Meier survival curves analysis showed that the survival time of patients with bladder urothelial carcinoma whose ex-pression of CD147 were high was significant lower than that of patients with bladder urothelial carcinoma whose expression of CD147 were low(P<0.01).Univariate analysis showed that histological grade,tumor stage,lymph node metastasis,tumor size and CD147 expression were all positively correlated with a poor prognosis(P<0.05).Cox-multivariate analysis revealed that histologi-cal grade,tumor stage,lymph node metastasis,and CD147 expression level were independent predictors of an unfavorable prognosis (P<0.05).Conclusion High expression of CD147 in bladder urothelial carcinoma may be correlate with the its progress and its expression state is an independent prognosis factor.