重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
35期
4784-4786
,共3页
杨晓华%李克伟%张广武%仝志琴%刘笑洁%李莹
楊曉華%李剋偉%張廣武%仝誌琴%劉笑潔%李瑩
양효화%리극위%장엄무%동지금%류소길%리형
手足口病%普通肠道病毒属%CA6
手足口病%普通腸道病毒屬%CA6
수족구병%보통장도병독속%CA6
hand-foot-and-mouth disease%common enterovirus%CA6
目的：了解洛阳市引起手足口病的普通肠道病毒的构成情况，警惕比例较高毒株发展为新的优势毒株，为洛阳市的手足口病防控提供科学依据。方法采用实时荧光PCR（RT‐PCR）对洛阳市手足口病的送检粪便标本进行核酸鉴定，随机选取9例普通肠道病毒阳性，而柯萨奇病毒A16型（CA16）和新肠道病毒71型（EV71）阴性的标本，进行5′UTR测序，确定毒株的亚型。利用CA6的特异性引物，选取75例普通肠道病毒阳性标本（CA16和EV71阴性），用 RT‐PCR方法鉴定CA6的阳性数量，分析CA6的构成比。结果9个普通肠道病毒5′UTR测序结果进行BLAST初筛，结果显示CA6为5株，在普通的肠道病毒中为优势毒株；75例普通肠道病毒中，共检测出CA620例。CA6在普通肠道病毒中的比例为29．76％（25／84）；25例CA6中，男13例，女12例；引发重症的病例为2例；城市地区5例，乡村20例。结论洛阳市引起手足口病的普通肠道病毒的构成较为复杂，在引起手足口病的普通肠道病毒中CA6病毒所占比例较高，对此应引起重视。
目的：瞭解洛暘市引起手足口病的普通腸道病毒的構成情況，警惕比例較高毒株髮展為新的優勢毒株，為洛暘市的手足口病防控提供科學依據。方法採用實時熒光PCR（RT‐PCR）對洛暘市手足口病的送檢糞便標本進行覈痠鑒定，隨機選取9例普通腸道病毒暘性，而柯薩奇病毒A16型（CA16）和新腸道病毒71型（EV71）陰性的標本，進行5′UTR測序，確定毒株的亞型。利用CA6的特異性引物，選取75例普通腸道病毒暘性標本（CA16和EV71陰性），用 RT‐PCR方法鑒定CA6的暘性數量，分析CA6的構成比。結果9箇普通腸道病毒5′UTR測序結果進行BLAST初篩，結果顯示CA6為5株，在普通的腸道病毒中為優勢毒株；75例普通腸道病毒中，共檢測齣CA620例。CA6在普通腸道病毒中的比例為29．76％（25／84）；25例CA6中，男13例，女12例；引髮重癥的病例為2例；城市地區5例，鄉村20例。結論洛暘市引起手足口病的普通腸道病毒的構成較為複雜，在引起手足口病的普通腸道病毒中CA6病毒所佔比例較高，對此應引起重視。
목적：료해락양시인기수족구병적보통장도병독적구성정황，경척비례교고독주발전위신적우세독주，위락양시적수족구병방공제공과학의거。방법채용실시형광PCR（RT‐PCR）대락양시수족구병적송검분편표본진행핵산감정，수궤선취9례보통장도병독양성，이가살기병독A16형（CA16）화신장도병독71형（EV71）음성적표본，진행5′UTR측서，학정독주적아형。이용CA6적특이성인물，선취75례보통장도병독양성표본（CA16화EV71음성），용 RT‐PCR방법감정CA6적양성수량，분석CA6적구성비。결과9개보통장도병독5′UTR측서결과진행BLAST초사，결과현시CA6위5주，재보통적장도병독중위우세독주；75례보통장도병독중，공검측출CA620례。CA6재보통장도병독중적비례위29．76％（25／84）；25례CA6중，남13례，녀12례；인발중증적병례위2례；성시지구5례，향촌20례。결론락양시인기수족구병적보통장도병독적구성교위복잡，재인기수족구병적보통장도병독중CA6병독소점비례교고，대차응인기중시。
Objective To investigatethe constituents of enteroviruse which can cause hand‐foot‐and‐mouth disease(HFMD) ,and prevent against the development of new prevailing strains and provide basis for prevention and control of HFMD in Luoyang city . Methods Reverse‐transcription polymerase chain reaction (RT‐PCR) was used to identify the type of RNA of stool specimen of HFMD cases in Luoyang ,to distinguish the subtype of strain ,9 enterovirus‐positive specimens which coxcackie virusA16(CA16)‐and enterovirus71(EV71)‐negative were selected randomly for 5′UTR sequencing .strain‐specific primers were used to detect posi‐tive samples in 75 CA16‐ and EV71‐negative but other enterovirus‐positive specimens by using real time RT‐PCR .Composition characteristics of CA6 were further analyzed .Results Primary screening was conducted with the results of 5′UTR sequencing of 9 commonent erovirus‐positive specimens and there were 5 CA6‐positive ,which were prevailing strains;there were 20 CA6‐positive of 75 commonenterovirus‐positive specimens ,the proportion of CA6‐positive in enterovirus‐positive specimens was 29 .76% (25/84) . Thirteen samples were collected from male and 12 from female ,2 cases resulted in severe symptoms ,5 from city and 20 from coun‐try .Conclusion The composition of commonent erovirus which results in HFMD was complex and the proportion of CA6 was higher ,which should be pay more attention .
