CAJ | 학술논문

目的：探讨Livin基因在结肠癌组织芯片中的表达及其与Bcl-2相关性。方法选取结肠癌外科手术患者60例，采用免疫组化MaxVisionTM二步法检测结肠癌组织芯片中Livin、Bcl-2基因的表达。另取切缘正常结肠组织芯片25例作对照。结果结肠癌组织芯片中Livin基因阳性表达率（63．33％）显著高于切缘正常的结肠组织芯片（24．00％）（χ2＝10．93，P＜0．01）。结肠癌组织芯片中Livin基因表达与性别、年龄、肿瘤大小、组织分化程度和淋巴结转移等病理特征无相关性（P＞0．05），与浸润深度、Duke’s分期和远处转移等密切相关（P＜0．05或P＜0．01）。结肠癌组织芯片中Bcl－2基因阳性表达率（68．33％）显著高于切缘正常结肠组织（4．00％）（χ2＝29．22，P＜0．01）。结肠癌组织芯片中Livin基因表达与Bcl－2基因存在明显的正相关（P＜0．01）。多因素Logistic回归分析提示，浸润深度、Duke’s分期和远处转移进入模型（P＜0．05）。结论 Livin基因在结肠癌组织芯片的发病中扮演重要角色，与其恶性侵袭、转移病理过程密切相关。 Livin基因与Bcl-2基因的异常表达可能在结肠癌癌变中起协调作用。
목적：탐토Livin기인재결장암조직심편중적표체급기여Bcl-2상관성。방법선취결장암외과수술환자60례，채용면역조화MaxVisionTM이보법검측결장암조직심편중Livin、Bcl-2기인적표체。령취절연정상결장조직심편25례작대조。결과결장암조직심편중Livin기인양성표체솔（63．33％）현저고우절연정상적결장조직심편（24．00％）（χ2＝10．93，P＜0．01）。결장암조직심편중Livin기인표체여성별、년령、종류대소、조직분화정도화림파결전이등병리특정무상관성（P＞0．05），여침윤심도、Duke’s분기화원처전이등밀절상관（P＜0．05혹P＜0．01）。결장암조직심편중Bcl－2기인양성표체솔（68．33％）현저고우절연정상결장조직（4．00％）（χ2＝29．22，P＜0．01）。결장암조직심편중Livin기인표체여Bcl－2기인존재명현적정상관（P＜0．01）。다인소Logistic회귀분석제시，침윤심도、Duke’s분기화원처전이진입모형（P＜0．05）。결론 Livin기인재결장암조직심편적발병중분연중요각색，여기악성침습、전이병리과정밀절상관。 Livin기인여Bcl-2기인적이상표체가능재결장암암변중기협조작용。
Objective To discuss expression of livin genes in colon cancer tissues chip and its correlation with Bcl-2 genes. Methods A total of 60 cases of patients with colon cancer, who chose to be treated with surgical operation in General Surgery Department were selected, and applied with immunohistochemical MaxVisionTM two step method to detect the expression of livin and Bcl-2 genesn in tissues chip. 25 cases of colon tissues chip with normal cutting edge were additionally chosen as control group. Results Positive livin genes expression rates in colon cancer tissues chip (63.33%) were much higher than those in colon tissues with normal cutting edge, which appeared statistical differences (24.00%) (χ2=10.93, P<0.01). Livin genes expression in colon cancer tissues chip had no obvious relevance with gen-der, age, tumor size, differentiation degree, lymphatic metastasis and other pathological characteristics (P>0.05), but ob-vious relevance with invasive depth, Duke’s stages, distant metastasis and other pathological characteristics (P<0.05 or P<0.01). Positive Bcl-2 genes expression rates in colon cancer tissues chip (68.33%)were much higher than those in colon tissues with normal cutting edge, which appeared statistical differences (4.00%) (χ2=29.22, P<0.01). Livin genes expres-sion was positively relatedto Bcl-2 genes in expression in colon cancer tissues chip (P<0.01). The invasive depth, Duke’s stages and distant metastasis were included in meta stasis of dinthe Logistic regression model for livin genes expression (P<0.05). Conclusion Livin genes play important roles in the disease process of colon cancer tissues chip and have close relevance with pathologic process of malignant invasion and transfer of tumor. The expression of Livin genes and Bcl-2 genes may play synergetic roles in process of colon cancer.