CAJ | 학술논문

目的：观察亚硒酸钠对人增生性瘢痕成纤维细胞体外增殖的影响。方法体外培养人增生性瘢痕成纤维细胞，取第4代细胞用CCK‐8检测成纤维细胞的增殖情况，分为试验组和对照组，试验组分为6组（A、B、C、D、E、F组），分别加入等量含2．5、5．0、10．0、20．0、40．0、80．0μmol／L浓度亚硒酸钠的10％胎牛血清培养基；对照组加入等量的10％胎牛血清培养基，分别在24、48、72、96 h用CCK‐8试剂盒检测细胞增殖情况；Live／dead试剂检测加入不同浓度亚硒酸钠24 h后细胞的存活情况；细胞免疫组织化学法检测加入不同浓度亚硒酸钠24 h后，细胞内Ⅰ、Ⅲ型胶原的表达情况。结果（1）亚硒酸钠在2．5～80．0μmol／L浓度范围内，在同一时间内随着浓度的增加，对成纤维细胞的抑制率逐步增加（P＜0．05）；（2）在相同浓度作用下随着时间的延长，亚硒酸钠对成纤维细胞的抑制率逐渐增加（P＜0．05）；（3）Live／dead试剂检测结果显示，随着亚硒酸钠浓度的增加，凋亡细胞数逐渐增加；（4）随着亚硒酸钠的浓度增加，成纤维细胞内Ⅰ、Ⅲ型胶原表达逐渐减弱。结论亚硒酸钠在体外能抑制人增生性瘢痕成纤维细胞的增殖，并且可以降低成纤维细胞内Ⅰ、Ⅲ型胶原的表达。
목적：관찰아서산납대인증생성반흔성섬유세포체외증식적영향。방법체외배양인증생성반흔성섬유세포，취제4대세포용CCK‐8검측성섬유세포적증식정황，분위시험조화대조조，시험조분위6조（A、B、C、D、E、F조），분별가입등량함2．5、5．0、10．0、20．0、40．0、80．0μmol／L농도아서산납적10％태우혈청배양기；대조조가입등량적10％태우혈청배양기，분별재24、48、72、96 h용CCK‐8시제합검측세포증식정황；Live／dead시제검측가입불동농도아서산납24 h후세포적존활정황；세포면역조직화학법검측가입불동농도아서산납24 h후，세포내Ⅰ、Ⅲ형효원적표체정황。결과（1）아서산납재2．5～80．0μmol／L농도범위내，재동일시간내수착농도적증가，대성섬유세포적억제솔축보증가（P＜0．05）；（2）재상동농도작용하수착시간적연장，아서산납대성섬유세포적억제솔축점증가（P＜0．05）；（3）Live／dead시제검측결과현시，수착아서산납농도적증가，조망세포수축점증가；（4）수착아서산납적농도증가，성섬유세포내Ⅰ、Ⅲ형효원표체축점감약。결론아서산납재체외능억제인증생성반흔성섬유세포적증식，병차가이강저성섬유세포내Ⅰ、Ⅲ형효원적표체。
Objective To observe the effects of sodium‐selenite on human hypertrophic scar fibroblasts proliferation in vitro . Methods Human hypertrophic scar fibroblast culture was conducted in vitro ,the status of fibroblast proliferation of the 4th gener‐ation cells was tested by CCK‐8 ,which was divided into experimental group and control group ,the experimental group was divided into six groups (A ,B ,C ,D ,E ,F) ,and were added an equal volume‐containing 2 .5 ,5 .0 ,10 .0 ,20 .0 ,40 .0 ,80 .0 μmol/L concentra‐tions of sodium selenite in 10% FBS culture medium ;the control group added an equal volume of 10% FBS culture medium ,testing cell proliferation by CCK‐8 at 24 ,48 ,72 ,96 h respectively ;testing different concentrations of sodium‐selenite cell survival situation after 24 h by Live/dead reagent ;immunohistochemical was used to test intracellularⅠ ,Ⅲ type collagen expression after 24 h .Re‐sults (1)With the increased of concentration ,the inhibition rate of fibroblasts gradually increased as the concentration of sodium‐selenite ranged in 2 .5-80 .0 μmol/L(P<0 .05);(2) the inhibition rate of sodium‐selenite on fibroblasts gradually increased at the same concentration with time(P<0 .05);(3)Live/dead reagent test results showed that apoptosis cell number increased with the concentration increasing ;(4 ) With concentrations of sodium‐selenite increasing ,typeⅠ ,Ⅲ collagen expression of fibroblast de‐creased gradually .Conclusion Sodium‐selenite can inhibit human hypertrophic scar fibroblast proliferate in vitro and reduce Ⅰ ,Ⅲcollagen expression of fibroblast type.