中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2014年
35期
1-4
,共4页
赵俞%余智%卢波达%刘凯的%方来明
趙俞%餘智%盧波達%劉凱的%方來明
조유%여지%로파체%류개적%방래명
吡格列酮%脑缺血再灌注损伤%TNF-α%IL-10%细胞凋亡
吡格列酮%腦缺血再灌註損傷%TNF-α%IL-10%細胞凋亡
필격렬동%뇌결혈재관주손상%TNF-α%IL-10%세포조망
Pioglitazone%Cerebral ischemia reperfusion%TNF-α%IL-10%Cell apoptosis
目的：探讨急性脑缺血再灌注损伤大鼠肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和细胞凋亡的变化规律及吡格列酮（Pioglitazone，PGZ）干预对上述指标的影响。方法将84只SD大鼠随机分为PGZ组（n=42）及对照组（n=42），前者通过改良Zea-longa法建立脑缺血再灌注损伤模型后立即经尾静脉注射10 mg/kg的PGZ，对照组则用同等剂量的生理盐水代替PGZ，以后各组分别每24小时腹腔注射1次等量PGZ/生理盐水，直至SD大鼠被处死，并以伤后处死时间分为7个亚组，分别为1h、3h、6h、12h、24h、3d和7d，每个亚组各6只。取缺血灶周围组织采用免疫组织化学方法检测并比较脑组织中TNF-α及IL-10的蛋白表达，同时采用TUNEL法观察并比较细胞凋亡情况。结果 PGZ组TNF-α蛋白表达量较对照组明显下降（P<0.05），而IL-10显著上升（P<0.05），且两组中二者均呈显著负相关（P<0.01）；同时PGZ组脑组织细胞凋亡数量较对照组也有所下降（P<0.05）。结论脑缺血再灌注损伤后，吡格列酮可能通过降低TNF-α的活性上调IL-10的表达，减轻炎症反应，阻止神经细胞进一步凋亡，从而对缺血再灌注损伤大鼠神经细胞发挥保护作用。
目的：探討急性腦缺血再灌註損傷大鼠腫瘤壞死因子-α（TNF-α）、白細胞介素-10（IL-10）和細胞凋亡的變化規律及吡格列酮（Pioglitazone，PGZ）榦預對上述指標的影響。方法將84隻SD大鼠隨機分為PGZ組（n=42）及對照組（n=42），前者通過改良Zea-longa法建立腦缺血再灌註損傷模型後立即經尾靜脈註射10 mg/kg的PGZ，對照組則用同等劑量的生理鹽水代替PGZ，以後各組分彆每24小時腹腔註射1次等量PGZ/生理鹽水，直至SD大鼠被處死，併以傷後處死時間分為7箇亞組，分彆為1h、3h、6h、12h、24h、3d和7d，每箇亞組各6隻。取缺血竈週圍組織採用免疫組織化學方法檢測併比較腦組織中TNF-α及IL-10的蛋白錶達，同時採用TUNEL法觀察併比較細胞凋亡情況。結果 PGZ組TNF-α蛋白錶達量較對照組明顯下降（P<0.05），而IL-10顯著上升（P<0.05），且兩組中二者均呈顯著負相關（P<0.01）；同時PGZ組腦組織細胞凋亡數量較對照組也有所下降（P<0.05）。結論腦缺血再灌註損傷後，吡格列酮可能通過降低TNF-α的活性上調IL-10的錶達，減輕炎癥反應，阻止神經細胞進一步凋亡，從而對缺血再灌註損傷大鼠神經細胞髮揮保護作用。
목적：탐토급성뇌결혈재관주손상대서종류배사인자-α（TNF-α）、백세포개소-10（IL-10）화세포조망적변화규률급필격렬동（Pioglitazone，PGZ）간예대상술지표적영향。방법장84지SD대서수궤분위PGZ조（n=42）급대조조（n=42），전자통과개량Zea-longa법건립뇌결혈재관주손상모형후립즉경미정맥주사10 mg/kg적PGZ，대조조칙용동등제량적생리염수대체PGZ，이후각조분별매24소시복강주사1차등량PGZ/생리염수，직지SD대서피처사，병이상후처사시간분위7개아조，분별위1h、3h、6h、12h、24h、3d화7d，매개아조각6지。취결혈조주위조직채용면역조직화학방법검측병비교뇌조직중TNF-α급IL-10적단백표체，동시채용TUNEL법관찰병비교세포조망정황。결과 PGZ조TNF-α단백표체량교대조조명현하강（P<0.05），이IL-10현저상승（P<0.05），차량조중이자균정현저부상관（P<0.01）；동시PGZ조뇌조직세포조망수량교대조조야유소하강（P<0.05）。결론뇌결혈재관주손상후，필격렬동가능통과강저TNF-α적활성상조IL-10적표체，감경염증반응，조지신경세포진일보조망，종이대결혈재관주손상대서신경세포발휘보호작용。
Objective To investigate the changes of TNF-α, IL-10 and cell apoptosis of cerebral ischemia-reperfusion injury and the influence of Pioglitazone(PGZ) on these parameters in rats. Methods Eighty-four male SD rats were ran-domly divided into two groups, PGZ group (n=42) and the control group (n=42). The PGZ group was treated with improved Zea-longa method and received tail vein injections of PGZ (10 mg/kg) immediately after injury, the control group re-ceived tail vein injections with the same dose sodium chloride injection immediately, after injury and repeat one time everyday until the rats was killed. Each group was divided into seven subgroups by sacrificed time after injury, those were 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, and 7 d, each subgroup got 6 rats. Each subgroup were randomly selected three rats after being killed, detected the expression of TNF-α and IL-10 of rats contusion peri tissues brain tissue by using im munohistochemical methods, while using TUNEL method to observe the peri cell apoptosis after brain contusion. Results The expression of TNF-α in each PGZ group was significantly decreased but the IL-10 was significant ly increased compared with the control group (P<0.05),and a significant negative correlation between the both of parameters in two groups as well (P<0.01);At the same time the number of apoptotic cells was decreasing (P<0.05). Conclusion Pioglita-zone is probably through the route of relieving inflammation response, reducing the change of secondary brain injury af-ter traumatic brain injury and decreasing neural cell apoptosis, and then provide protection of neurocytes.
