CAJ | 학술논문

目的：探讨豚鼠乳鼠心房肌细胞培养的条件和方法。方法取1～2 d 龄豚鼠乳鼠的心房肌，用0．06％的胰蛋白酶消化1次，再用0．08％Ⅱ型胶原酶重复消化4次，并采用前2次、后2次和前后4次消化后的细胞分别培养法，观察3者对心房肌细胞产量、存活率、搏动率及纯化率的影响。结果3种培养法都得到可观的心房肌细胞数量和较高的存活率、搏动率及纯化率，特别是后2次消化后的细胞培养法。结论应用0．06％的胰蛋白酶消化1次和0．08％Ⅱ型胶原酶重复消化4次，可以分离出数量较多、状态良好的心房肌细胞。
목적：탐토돈서유서심방기세포배양적조건화방법。방법취1～2 d 령돈서유서적심방기，용0．06％적이단백매소화1차，재용0．08％Ⅱ형효원매중복소화4차，병채용전2차、후2차화전후4차소화후적세포분별배양법，관찰3자대심방기세포산량、존활솔、박동솔급순화솔적영향。결과3충배양법도득도가관적심방기세포수량화교고적존활솔、박동솔급순화솔，특별시후2차소화후적세포배양법。결론응용0．06％적이단백매소화1차화0．08％Ⅱ형효원매중복소화4차，가이분리출수량교다、상태량호적심방기세포。
Objective To explore the condition and method of primary cultures of atrial myoeytes in sukling guinea pig .Methods The atrial myoeytes of sukling guinea pig with 1‐2 d old were taken and digested once by 0 .06% trypsin ,then repeatedly digested by 0 .08% type Ⅱ collagenase for four times .And the cells after former twice ,latter twice and former and latter four times of diges‐tion were adopted for conducting respective culture .The influence of three kinds of culture method on atrial myocytes production , survival rate ,pulsating rate and purification rate were observed .Results Three kinds of culture method all obtained the considera‐ble number of atrial myocytes and a higher survival rate ,pulsation rate and purification rate ,particularly the cell culture method af‐ter latter twice digestion .Conclusion Once digestion by 0 .06% trypsin and 0 .08% type Ⅱ collagenase for four times can isolate a great quantity and good condition of atrial myocytes .