护理研究
護理研究
호리연구
NURSING RESEARCH
2015年
1期
18-21
,共4页
冷热护理%宫颈癌%H eLa细胞%细胞克隆%护理干预
冷熱護理%宮頸癌%H eLa細胞%細胞剋隆%護理榦預
랭열호리%궁경암%H eLa세포%세포극륭%호리간예
hot and cold care%cervical cancer%HeLa cell%cell clone%nursing intervention
[目的]研究冷热护理刺激对人宫颈癌 H eLa细胞克隆形成能力的影响及机制,以期探索冷热护理的新作用机制,促进临床护理水平的提高。[方法]HeLa细胞按常规用DMEM 高糖培养基培养；按6孔板每孔100个~300个细胞的浓度接种并在不同培养温度情况下进行细胞克隆试验；提取在不同温度环境下生长的同批次 HeLa细胞的总 RNA,在逆转录反应后获得 cDNA,以此为模板,实施荧光定量分析不同温度环境下 pan QKI mRNA的表达水平。[结果]较高的环境温度能增强 HeLa细胞的克隆形成能力,抑制 pan QKI mRNA表达；但较低的环境温度则抑制了该细胞的克隆形成,同时,pan QKI mRNA表达水平显著增高。[结论]温度干预能对人宫颈癌 HeLa细胞的克隆能力产生影响,pan QKI 在不同温度环境中的表达变化可能是导致 HeLa细胞克隆形成能力差别的重要原因,温度干预的护理效用和机制值得深入探究。
[目的]研究冷熱護理刺激對人宮頸癌 H eLa細胞剋隆形成能力的影響及機製,以期探索冷熱護理的新作用機製,促進臨床護理水平的提高。[方法]HeLa細胞按常規用DMEM 高糖培養基培養；按6孔闆每孔100箇~300箇細胞的濃度接種併在不同培養溫度情況下進行細胞剋隆試驗；提取在不同溫度環境下生長的同批次 HeLa細胞的總 RNA,在逆轉錄反應後穫得 cDNA,以此為模闆,實施熒光定量分析不同溫度環境下 pan QKI mRNA的錶達水平。[結果]較高的環境溫度能增彊 HeLa細胞的剋隆形成能力,抑製 pan QKI mRNA錶達；但較低的環境溫度則抑製瞭該細胞的剋隆形成,同時,pan QKI mRNA錶達水平顯著增高。[結論]溫度榦預能對人宮頸癌 HeLa細胞的剋隆能力產生影響,pan QKI 在不同溫度環境中的錶達變化可能是導緻 HeLa細胞剋隆形成能力差彆的重要原因,溫度榦預的護理效用和機製值得深入探究。
[목적]연구랭열호리자격대인궁경암 H eLa세포극륭형성능력적영향급궤제,이기탐색랭열호리적신작용궤제,촉진림상호리수평적제고。[방법]HeLa세포안상규용DMEM 고당배양기배양；안6공판매공100개~300개세포적농도접충병재불동배양온도정황하진행세포극륭시험；제취재불동온도배경하생장적동비차 HeLa세포적총 RNA,재역전록반응후획득 cDNA,이차위모판,실시형광정량분석불동온도배경하 pan QKI mRNA적표체수평。[결과]교고적배경온도능증강 HeLa세포적극륭형성능력,억제 pan QKI mRNA표체；단교저적배경온도칙억제료해세포적극륭형성,동시,pan QKI mRNA표체수평현저증고。[결론]온도간예능대인궁경암 HeLa세포적극륭능력산생영향,pan QKI 재불동온도배경중적표체변화가능시도치 HeLa세포극륭형성능력차별적중요원인,온도간예적호리효용화궤제치득심입탐구。
Objective:To probe into the influence of hot and cold care stimulus on forming ability of human cervi-cal cancer HeLa cells clone and its mechanism,so as to explore the new action mechanism of hot and cold care promote the improvement of clinical nursing level.Methods:HeLa cells were cultured by routine use of DMEM high glucose medium.According the concentration of 100~300 cells per hole,they were inoculated in 6 hole plate,cell cloning experiments were carried out in different culture temperature circumstances;the total RNA of the same batch of HeLa cells was extracted which grew in different temperatures,the cDNA obtained in the re-verse transcription reaction was taken as a template,the fluorescent quantitative analysis was implemented for expression level of pan QKI mRNA under different temperature environment.Results:The high ambient tem-perature can enhance the colony formation ability of HeLa cells and inhibit the pan QKI mRNA expression;but the lower ambient temperature inhibited the cell clone formation,at the same time,the expression level of mRNA was significantly increased in pan QKI.Conclusion:Temperature intervention can affect cloning ability of human cervical cancer HeLa cells.The changes of pan QKI expression in different temperature environ-ments may be an important reason of differences of HeLa cell clone forming ability.The efficacy and mechanism of temperature intervention are worth for in depth inquiry.
