CAJ | 학술논문

研究施加外源硒(Se6+)对苎麻镉胁迫毒性的保护机制。主要讨论硒对苎麻生长、MDA、抗氧化酶活性以及DNA甲基化水平的影响。苎麻分别在含镉浓度为0、3、6、9 mg/L条件下培养，并对苎麻叶面喷洒硒酸钠(1.2μmol/L)。结果表明，镉浓度为3、6、9 mg/L营养液中培养的苎麻，经过向叶片喷洒低浓度的硒酸钠(1.2μmol/L)处理后，苎麻叶片中的SOD活性增加了35.34%、43.18%、3.63%，POD活性增加了12.45%、14.14%、3.27%， DNA 甲基化下降了10.70%、18.18%、15.59%。结果证实了低浓度硒能强化苎麻 SOD、POD 活性以及平衡苎麻体内DNA甲基化。
연구시가외원서(Se6+)대저마력협박독성적보호궤제。주요토론서대저마생장、MDA、항양화매활성이급DNA갑기화수평적영향。저마분별재함력농도위0、3、6、9 mg/L조건하배양，병대저마협면분쇄서산납(1.2μmol/L)。결과표명，력농도위3、6、9 mg/L영양액중배양적저마，경과향협편분쇄저농도적서산납(1.2μmol/L)처리후，저마협편중적SOD활성증가료35.34%、43.18%、3.63%，POD활성증가료12.45%、14.14%、3.27%， DNA 갑기화하강료10.70%、18.18%、15.59%。결과증실료저농도서능강화저마 SOD、POD 활성이급평형저마체내DNA갑기화。
The protective role of exogenously supplied selenium (Se6+) on Bechmeria nivea (L.) Gaud (Ramie) subjected to cadmium (Cd) stress was studied in vitro, and the mechanism was discussed by investigating plant growth, malondialdehyde (MDA), activity of antioxidative enzymes and DNA methylation pattern. Plants grown in hydroponic culture were supplied with spraying Se (selenate, 1.2μmol/L) and cadmium (Cd(NO3)2, 0, 3, 6 and 9 mg/L), individually or simultaneously. At low Se spraying levels, SOD activity was increased by 35.34%, 43.18%, 3.63%under 3, 6 or 9 mg/L cadmium contents, POD was increased by 12.45%, 14.14%, 3.27%, and the level of DNA methylation was decreased by 10.70%, 18.18%and 15.59%, respectively. The results confirmed that spraying low Se on ramie leaves could enhance the activity of SOD and POD, and regulate DNA methylation in ramie leaves.