CAJ | 학술논문

目的：探讨胰岛素类似物生长因子1受体（IGF1R）基因沉默对人类肾上腺皮质癌细胞株SW13增殖及凋亡的影响。方法依据人类IGF1R的cDNA序列合成其特异性SiRNA ，通过脂质体将其转入SW13细胞，24 h后通过实时定量Real time PCR检测SiRNA对靶基因mRNA下调作用，同时使用Western blot检测其靶蛋白的表达。通过MTT法测定转染后细胞增殖活性，流式细胞仪测定细胞凋亡状态。结果3种不同的针对 IGF1R的SiRNA 能够有效下调SW13细胞 IGF1R mRNA ，而通过Western blot也发现在蛋白水平出现类似结果。IGF1R基因沉默能够有效抑制SW13细胞增殖以及诱导细胞凋亡。结论IGF1R基因沉默能够诱导肾上腺皮质癌细胞凋亡，抑制其增殖，有望为肿瘤靶向治疗在ACC中应用提供相应的理论依据。
목적：탐토이도소유사물생장인자1수체（IGF1R）기인침묵대인류신상선피질암세포주SW13증식급조망적영향。방법의거인류IGF1R적cDNA서렬합성기특이성SiRNA ，통과지질체장기전입SW13세포，24 h후통과실시정량Real time PCR검측SiRNA대파기인mRNA하조작용，동시사용Western blot검측기파단백적표체。통과MTT법측정전염후세포증식활성，류식세포의측정세포조망상태。결과3충불동적침대 IGF1R적SiRNA 능구유효하조SW13세포 IGF1R mRNA ，이통과Western blot야발현재단백수평출현유사결과。IGF1R기인침묵능구유효억제SW13세포증식이급유도세포조망。결론IGF1R기인침묵능구유도신상선피질암세포조망，억제기증식，유망위종류파향치료재ACC중응용제공상응적이론의거。
Objective To investigate the effect of IGF1R gene silencing by SiRNA on the proliferation and apoptosis in human adrenocortical carcinoma cell line SW13 .Methods The specific SiRNA oligos for human IGF1R were synthesized , and transfected into SW13 cells by lipofectamine 2000 .The expression of IGF1R mRNA was detected by Realtime PCR after being incubated for 24 h .Western blot was used to detect the IGF1R protein .The proliferation and apoptosis of SW13 cells were evaluated by MTT and flow cytometry respectively .Results The inhibition efficiency of SiRNA for IGF 1R in the mR‐NA level was high and the same result was observed in the protein level by Western blot .MTT and flow cytometry showed that IGF1R gene silencing might lead to the inhibition of cell growth and enhancement of cell apoptosis .Conclusion IGF1R gene silencing of SW13 cells may induce apoptosis and inhibit proliferation of these cells .