CAJ | 학술논문

用高效液相色谱法测定桑葚中芦丁的含量，色谱柱Agilent-C18（250 mm ×4.6 mm，5μm），流动相为甲醇-0.5%磷酸（33：67），流速1.0 mL/min，检测波长358 nm，进样量20μL，柱温30℃。芦丁在0.0239~0.1197 mg/mL浓度范围内与色谱峰面积呈良好的线性关系（ r=0.9998）。该方法灵敏度高，专属性强，重现性好，适合于桑葚药用的质量控制。
용고효액상색보법측정상심중호정적함량，색보주Agilent-C18（250 mm ×4.6 mm，5μm），류동상위갑순-0.5%린산（33：67），류속1.0 mL/min，검측파장358 nm，진양량20μL，주온30℃。호정재0.0239~0.1197 mg/mL농도범위내여색보봉면적정량호적선성관계（ r=0.9998）。해방법령민도고，전속성강，중현성호，괄합우상심약용적질량공제。
To establish an HPLC method for the determination of rutin in Mori Fructus. The chromato-graphic condition included a Agilent-C18(250 mm × 4. 6 mm,5 μm)column and the mobile phase con-sisting of methanol-0. 5% phosphate acid(33:67),the detection wavelength was at 358 nm and the flow rate was 1. 0 mL/min,the inkection volume was 20μL and the colum temperature was 30℃. The calibra-tion curve of rutin showed good linearity(r=0. 999 8)in the range of 0. 023 9~0. 119 7 mg/mL. The method has high sensitivity,strong specificity,good reproducibility,and can be used for control of rutin in Mori Fructus.