应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2014年
12期
2292-2294,2297
,共4页
杨阳%刘少静%杨光华%赵长琦
楊暘%劉少靜%楊光華%趙長琦
양양%류소정%양광화%조장기
蓝花喜盐鸢尾%异黄酮糖苷%鸢尾甲黄素 B4ˊ-O-β-D-葡萄糖苷%反相高效液相色谱法
藍花喜鹽鳶尾%異黃酮糖苷%鳶尾甲黃素 B4ˊ-O-β-D-葡萄糖苷%反相高效液相色譜法
람화희염연미%이황동당감%연미갑황소 B4ˊ-O-β-D-포도당감%반상고효액상색보법
iris halOphila var. sOGdiaNa%isoflavone glycoside%iristectorigenin B 4ˊ-O-β-D-glucopyrano-side%RP-HPLC
建立简单、快速的HPLC法测定蓝花喜盐鸢尾中一种全新异黄酮糖苷———鸢尾甲黄素B 4ˊ-O-β-D-葡萄糖苷。采用HPLC法,Agilent 1260HC-C18(4.6 mm ×250 mm,5μm)色谱柱,流动相为甲醇-1%冰醋酸梯度洗脱,流速为0.9 mL/min,检测波长为268 nm,柱温为25℃。结果表明,该异黄酮糖苷在17.2~172μg/mL浓度内线性关系良好,相关系数为0.9994;回收率为99.78%(RSD=0.76%)。该检测方法准确灵敏,适用于蓝花喜盐鸢尾中鸢尾甲黄素B4ˊ-O-β-D-葡萄糖苷的测定。
建立簡單、快速的HPLC法測定藍花喜鹽鳶尾中一種全新異黃酮糖苷———鳶尾甲黃素B 4ˊ-O-β-D-葡萄糖苷。採用HPLC法,Agilent 1260HC-C18(4.6 mm ×250 mm,5μm)色譜柱,流動相為甲醇-1%冰醋痠梯度洗脫,流速為0.9 mL/min,檢測波長為268 nm,柱溫為25℃。結果錶明,該異黃酮糖苷在17.2~172μg/mL濃度內線性關繫良好,相關繫數為0.9994;迴收率為99.78%(RSD=0.76%)。該檢測方法準確靈敏,適用于藍花喜鹽鳶尾中鳶尾甲黃素B4ˊ-O-β-D-葡萄糖苷的測定。
건립간단、쾌속적HPLC법측정람화희염연미중일충전신이황동당감———연미갑황소B 4ˊ-O-β-D-포도당감。채용HPLC법,Agilent 1260HC-C18(4.6 mm ×250 mm,5μm)색보주,류동상위갑순-1%빙작산제도세탈,류속위0.9 mL/min,검측파장위268 nm,주온위25℃。결과표명,해이황동당감재17.2~172μg/mL농도내선성관계량호,상관계수위0.9994;회수솔위99.78%(RSD=0.76%)。해검측방법준학령민,괄용우람화희염연미중연미갑황소B4ˊ-O-β-D-포도당감적측정。
To establish a simple,rapid HPLC method for determination of a novel isoflavone glycoside iris-tectorigenin B 4ˊ-O-β-D-glucopyranoside in iris halOphila var. sOGdiaNa. HPLC separation was carried out with an Agilent 1260HC-C18 column( 4. 6 mm × 250 mm,5 μm)by gradient elution using methanol-1%acetic acid as mobile phase at a flow rate of 0. 9 mL/min. The detection wavelength was 268 nm and the column temperature was maintained at 25℃. Results showed that good linear relationship was observed in the range of 17. 2~172 μg/mL(R2 =0. 999 4). The recovery rate was 99. 78%(RSD=0. 76%). The method has the advantages of acuteness and precision,which is suitable for determination of iristectorige-nin B 4ˊ-O-β-D-glucopyranoside in iris halOphila var. sOGdiaNa.