CAJ | 학술논문

目的：建立毒鼠强中毒患者的胃内容物、血液和尿液中快速检测毒鼠强的气质联用方法。方法运用C18小柱对胃内容物与血液进行除杂、净化后,通过气质联用仪上的大体积进样与程序升温进样,并利用小口径毛细管色谱柱HP-5MS(0.18 mm×20 m,0.18μm)进行检测,用选择离子模式对m/z92、132、212、240进行采集。结果从样品前处理到确证完成全过程耗时约20 min。结论该方法具操作简易快速、定性准确、灵敏度高等特点,能应用于应急检验,为患者抢救工作节省了宝贵的时间。
목적：건립독서강중독환자적위내용물、혈액화뇨액중쾌속검측독서강적기질련용방법。방법운용C18소주대위내용물여혈액진행제잡、정화후,통과기질련용의상적대체적진양여정서승온진양,병이용소구경모세관색보주HP-5MS(0.18 mm×20 m,0.18μm)진행검측,용선택리자모식대m/z92、132、212、240진행채집。결과종양품전처리도학증완성전과정모시약20 min。결론해방법구조작간역쾌속、정성준학、령민도고등특점,능응용우응급검험,위환자창구공작절성료보귀적시간。
Objective To establish a gas chromatography-mass spectrometry selected ion monitoring method for the determination of tetramine in the gastric content, the blood or the urine from the poisoned patients.Methods The C18-solid phase extraction column was adopt to purify the substrate of the gastric contents or the blood. Large volume injection of samples with a programmed temperature vaporize (PTV) system were discussed. With the conditions of small diameter capillary chromatographic column (HP-5MS: 20 m×0.18 mm, 0.18μm), selected ion monitoring (SIM) acquired mode were selected form/z92, 132, 212, and 240.Results The whole process took about 20 min. Conclusion This method is rapid, accurate, and sensitive. It can be applied to emergency inspection, and save precious time for the patient in the tetramine poisoning rescue work.