CAJ | 학술논문

目的：从海藻多酚提取物中筛选细菌群体感应抑制剂并研究其活性。方法采用紫色杆菌Chromo-bacteriumviolaceum CV026琼胶扩散法及高效液相色谱(high performance liquid chromatography, HPLC)法检测海藻多酚群体感应抑制活性及其成分组成。结果在选取的25种海藻中,12种海藻多酚具有群体感应抑制活性,其中石莼、马尾藻、裙带菜多酚的抑制活性最强。在1.0 mg/mL多酚浓度下,其形成的群体感应抑制圈直径分别为25.18、21.61、20.43 mm。采用HPLC进一步对三种海藻多酚的成分进行检测,结果显示三种海藻多酚均含有儿茶酚及表儿茶素。此外,还含有少量的阿魏酸及芦丁。结论石莼、马尾藻、裙带菜多酚具有群体感应抑制活性,有望开发为新型群体感应抑制剂。
목적：종해조다분제취물중사선세균군체감응억제제병연구기활성。방법채용자색간균Chromo-bacteriumviolaceum CV026경효확산법급고효액상색보(high performance liquid chromatography, HPLC)법검측해조다분군체감응억제활성급기성분조성。결과재선취적25충해조중,12충해조다분구유군체감응억제활성,기중석순、마미조、군대채다분적억제활성최강。재1.0 mg/mL다분농도하,기형성적군체감응억제권직경분별위25.18、21.61、20.43 mm。채용HPLC진일보대삼충해조다분적성분진행검측,결과현시삼충해조다분균함유인다분급표인다소。차외,환함유소량적아위산급호정。결론석순、마미조、군대채다분구유군체감응억제활성,유망개발위신형군체감응억제제。
ObjectiveTo evaluate the quorum sensing inhibitors activity of marine algae polyphenols. Methods Quorum sensing (QS) inhibition was measured by qualitative agar diffusion assay using the bacterial model ofChromobacterium violaceum CV026. The presence of phenolic compounds were detected by high performance liquid chromatography (HPLC).ResultsTwelve out of 25 tested algae polyphenols possessed QS inhibitors activity. Polyphenols extracted fromUlva lactucal, Sargassum vachellianum and Undaria pinnatifida significantly reduced violacein production of C. violaceum CV026 at the concentration of 1.0 mg/mL, and the diameter of inhibitory violacein was 25.18, 21.61 and 20.43 mm, respectively. The presence of phenolic compounds were detected by HPLC, which revealed that the reduced violacein production of C. violaceum CV026 might be due to the high content of catechin and epicatechin with respect to their quorum sensing inhibitors activity. ConclusionThe results suggested the inhibiting QS system of bacteria using algae polyphones could lead to the development of new quorum sensing inhibitors.