CAJ | 학술논문

目的：分离和筛选出一株高产 D-阿拉伯醇菌株。方法采用初筛和复筛手段,利用纸层析法和高效液相检测方法,从具有高浓度葡萄糖环境,如葡萄干、红薯地土壤、蜂蜜、花粉等含糖量较高的样品中分离和筛选得到了一株高效转化葡萄糖生成D-阿拉伯醇的优势菌株。结果该菌株在初始葡萄糖浓度为200 g/L的培养基中,30℃,摇瓶培养120 h,D-阿拉伯醇的产量高达82.4 g/L。根据常规形态鉴别,生理生化实验测定,以及核酸序列同源性比较分析,证实筛选出的高产菌株b-1属于Candida parapsilosis。结论优势菌株b-1发酵产D-阿拉伯醇的产率较高,副产物较少,具有一定的工业化应用前景。
목적：분리화사선출일주고산 D-아랍백순균주。방법채용초사화복사수단,이용지층석법화고효액상검측방법,종구유고농도포도당배경,여포도간、홍서지토양、봉밀、화분등함당량교고적양품중분리화사선득도료일주고효전화포도당생성D-아랍백순적우세균주。결과해균주재초시포도당농도위200 g/L적배양기중,30℃,요병배양120 h,D-아랍백순적산량고체82.4 g/L。근거상규형태감별,생리생화실험측정,이급핵산서렬동원성비교분석,증실사선출적고산균주b-1속우Candida parapsilosis。결론우세균주b-1발효산D-아랍백순적산솔교고,부산물교소,구유일정적공업화응용전경。
Objective To isolate and screen a high yield strain forD-arabitol production.Methods With the determination of paper chromatography and high performance liquid chromatography (HPLC), the fine strain for high concentration yield ofD-arabitol from glucose had been separated and screened from high glucose environment, such as raisins, sweet potato to soil, honey, pollen samples, after the first and second screening.Results At the initial glucose concentration of 200 g/L on the culture medium, 30℃,D-arabitol yield was 82.4 g/L after the fermentation of 120 h. According to the conventional morphological identification, determination of physiological and biochemical experiment, and the nucleic acid sequence homology comparison analysis, the high-producing strain b-1 was confirmed asCandida parapsilosis.Conclusion The yield ofD-arabitol is high, the by-products are low in the fermentation by the screened strains b-1, which has a certain application prospect of industrialization.