CAJ | 학술논문

噬菌体展示技术是一种特殊的基因工程重组表达技术，已经作为一种强大的工具运用在不同的研究中，包括筛选抗体和酶的配体、筛选小分子的受体、基因工程抗体等。免疫分析作为农药残留快速筛查技术被广泛研究，利用抗体从噬菌体展示肽库中淘选竞争物，可以加快异源免疫分析方法的建立；利用抗原抗体复合物从噬菌体展示肽库中淘选抗免疫复合体多肽，可建立非竞争免疫分析方法，丰富农药的免疫分析模式。本文从噬菌体展示技术的原理、噬菌体展示肽库的构建和噬菌体展示肽库的筛选方面概述了噬菌体展示肽库技术；从模拟表位筛选、抗免疫复合体筛选和新免疫分析方法方面阐述了噬菌体展示肽库技术在农药等小分子免疫分析中的应用；并对噬菌体展示肽库技术的研究和应用前景进行了展望。
서균체전시기술시일충특수적기인공정중조표체기술，이경작위일충강대적공구운용재불동적연구중，포괄사선항체화매적배체、사선소분자적수체、기인공정항체등。면역분석작위농약잔류쾌속사사기술피엄범연구，이용항체종서균체전시태고중도선경쟁물，가이가쾌이원면역분석방법적건립；이용항원항체복합물종서균체전시태고중도선항면역복합체다태，가건립비경쟁면역분석방법，봉부농약적면역분석모식。본문종서균체전시기술적원리、서균체전시태고적구건화서균체전시태고적사선방면개술료서균체전시태고기술；종모의표위사선、항면역복합체사선화신면역분석방법방면천술료서균체전시태고기술재농약등소분자면역분석중적응용；병대서균체전시태고기술적연구화응용전경진행료전망。
Phage display technology (PDT) is a unique genetic engineering recombinant expression technology, which is a powerful tool for a variety of applications, including antibody engineering and the isolation of peptide ligands for antibodies and enzymes and peptide receptors for small molecules. Immunoassay had been widely studied as a rapid screening technique for pesticide residue, isolation of competitor from phage display peptide library by using antibody could accelerate the development of heterologous immunoassay; isolation of anti-immunocomplex peptide from phage display peptide library by using antigen-antibody complexes could develop noncompetitive immunoassay, and enriched immunoassay model for pesticide. This review concisely summarized phage display peptide library technology from the principle of phage display technology, the construction of phage display peptide library and screening of phage display peptide library. Appling phage display peptide library technology in immunoassay for pesticides and other small molecules were elaborated from screening of mimicking epitope, screening of anti-immunocomplex and the new immunoassays. Furthermore, this review presented insights for the study and application prospect of the phage display peptide library technology.