CAJ | 학술논문

目的：建立高效液相色谱-串联质谱法(HPLC-MS/MS)检测草莓中3-吲哚丁酸、赛苯隆、氯吡脲、3-吲哚乙酸、脱落酸5种植物生长调节剂的方法。方法样品采用QuEChERS方法乙腈提取， PSA吸附剂净化，高效液相色谱-串联质谱电喷雾(ESI)，多反应监测(MRM)负离子模式分析检测，外标法定量。结果各化合物在0.01~1.0 mg/L范围内均呈现良好的线性关系，相关系数均大于0.998。5种化合物的检出限在1~5μg/kg。添加回收率为72.0%~99.4%。相对标准偏差(RSD)均小于10%。结论通过线性、准确度、精密度实验及样品测定，验证了以水溶解样品、乙腈提取、QuEChERS净化的方法简便可行。
목적：건립고효액상색보-천련질보법(HPLC-MS/MS)검측초매중3-신타정산、새분륭、록필뇨、3-신타을산、탈락산5충식물생장조절제적방법。방법양품채용QuEChERS방법을정제취， PSA흡부제정화，고효액상색보-천련질보전분무(ESI)，다반응감측(MRM)부리자모식분석검측，외표법정량。결과각화합물재0.01~1.0 mg/L범위내균정현량호적선성관계，상관계수균대우0.998。5충화합물적검출한재1~5μg/kg。첨가회수솔위72.0%~99.4%。상대표준편차(RSD)균소우10%。결론통과선성、준학도、정밀도실험급양품측정，험증료이수용해양품、을정제취、QuEChERS정화적방법간편가행。
Objective Toestablisha method for the determination of 5 kinds of plant growth regulators (PGRs): 4-(3-Indolyl)-butyric acid (BAA), thidazuron, forchlorfenuron (CPPU), 3-Indolyl-acetic acid (ZAA) and abcisic acid (ABA) in food of plant origin using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).MethodsSamples were extracted by acetonitrile, and cleaned up by QuEChERS. Identification and determination were achieved by multiple reaction monitoring in negative mode using HPLC-MS/MS. The quantification of analytes was carried out by the external standard method.Results All the target compounds had a good linear relationship in the range of 0.01~1.00 mg/L. The relative coefficients were larger than 0.998. The LOQ of the tested analytes varied was between 1~5μg/kg. The recoveries were 72.0%~99.4%. The relative standard deviations (RSD) were less than 10%.Conclusion The method was proved to be simple and accurate in practice.