CAJ | 학술논문

序列特异性核酸体外扩增技术(nucleic acid specific-based amplification, NASBA)是在PCR基础上发展起来的一种扩增RNA的新技术,作为一种新型研究手段,具有便捷、准确性好、灵敏度高、周期短的特点,尤其适用于RNA的分析研究。核酸分析技术是出入境检验检疫工作的重要手段,可用于食品病原微生物、动植物产品中的有害生物、病原的分析及鉴定。本文简要介绍了NASBA技术的基本原理,在论证对比NASBA技术与普通PCR方法、荧光PCR方法及其他恒温扩增等核酸分析技术的差异及相似性后,根据其使用特点进一步对NASBA在进出境检验检疫的食品安全检测及动植物检疫的应用予以综述及展望。
서렬특이성핵산체외확증기술(nucleic acid specific-based amplification, NASBA)시재PCR기출상발전기래적일충확증RNA적신기술,작위일충신형연구수단,구유편첩、준학성호、령민도고、주기단적특점,우기괄용우RNA적분석연구。핵산분석기술시출입경검험검역공작적중요수단,가용우식품병원미생물、동식물산품중적유해생물、병원적분석급감정。본문간요개소료NASBA기술적기본원리,재론증대비NASBA기술여보통PCR방법、형광PCR방법급기타항온확증등핵산분석기술적차이급상사성후,근거기사용특점진일보대NASBA재진출경검험검역적식품안전검측급동식물검역적응용여이종술급전망。
Nucleic acid specific-based amplification (NASBA) is a new technology to amplify RNA originated in PCR. As a new research means, NASBA has the characteristics of convenience, good accuracy, high sensitivity and short periods, especially applicable to RNA analysis. The nucleic acid analysis technology is an important means in entry-exit inspection and quarantine, which can be used to detect and identify pathogenic microorganism in food, pests and pathogen in animals and plants. The paper briefly introduced basic principle of NASBA, compared NASBA to RT-PCR, realtime RT-PCR and other isothermal amplification methods and revealed their differences and similarity. According to the usage characteristics of NASBA, the paper reviewed the application and prospect of NASBA in food safety detection and animal and plant quarantine in entry-exit inspection and quarantine.