CAJ | 학술논문

目的：简化测定胶原蛋白粉中呋喃类代谢物残留的实验过程，缩短实验的检测时间。方法在GB/T21311-2007的基础上，对其进行方法改进，建立高效液相色谱-串联质谱法(HPLC-MS/MS)测定胶原蛋白粉中呋喃西林(SEM)、呋喃妥因(AHD)、呋喃唑酮(AOZ)、呋喃它酮(AMOZ)等4种呋喃代谢物的快速检测方法。采用55℃衍生化，对衍生品按照离心、调pH值、离心的步骤进行处理，使得调节pH值更容易，同时避免传统试验方法中使用分液漏斗时样品乳化成胶状体而不能达到液液分离的目的的现象。结果实验结果表明，本方法与《GB/T21311-2007动物源性食品中硝基呋喃类药物代谢物残留量检测方法高效液相色谱串联质谱法》相比，结果并无很大的差异，但是大大缩短了实验的检测时间、简化了实验过程。结论本实验方法更加适合大批量样品的试验检测。
목적：간화측정효원단백분중부남류대사물잔류적실험과정，축단실험적검측시간。방법재GB/T21311-2007적기출상，대기진행방법개진，건립고효액상색보-천련질보법(HPLC-MS/MS)측정효원단백분중부남서림(SEM)、부남타인(AHD)、부남서동(AOZ)、부남타동(AMOZ)등4충부남대사물적쾌속검측방법。채용55℃연생화，대연생품안조리심、조pH치、리심적보취진행처리，사득조절pH치경용역，동시피면전통시험방법중사용분액루두시양품유화성효상체이불능체도액액분리적목적적현상。결과실험결과표명，본방법여《GB/T21311-2007동물원성식품중초기부남류약물대사물잔류량검측방법고효액상색보천련질보법》상비，결과병무흔대적차이，단시대대축단료실험적검측시간、간화료실험과정。결론본실험방법경가괄합대비량양품적시험검측。
Objective To simplify the experimental process for determination of furan metabolite residues in collagen powder, and shorten the detection time of the experiment.Methods Based on GB/T21311-2007, the method was improved, and a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to rapidly determine 4 kinds of nitrofuran metabolite residues in collagen powder, including nitrofurazone (SEM), nitrofurantoin (AHD), furaltadone (AOZ) and furazolidone (AMOZ). After derivation at 55℃, the derivatives were processed following the steps of centrifugation, pH adjustment, and centrifugation again, making it easier to adjust the pH value, while avoiding the problem found in the conventional test method using a separatory funnel where the sample emulsified into colloid and could not achieve the purpose of liquid-liquid separation.Results The experimental results showed no significant differences between the proposed method and the national standard GB/T21311-2007, while the detection time of the experiment was greatly reduced, and the experimental process was simplified.Conclusion This method is more suitable for experimental detection of large quantities of samples.