CAJ | 학술논문

目的：建立对熟肉制品中日落黄、胭脂红和诱惑红三种合成色素同时测定的方法。方法样品采用无水乙醇-氨水-水(7：2：1)提取，加正己烷去除脂肪，氮吹后定容，用高效液相色谱法测定。经Symmetry Shield RP18柱分离，甲醇-0.02 mol/L乙酸铵溶液梯度洗脱，检测波长为508 nm。结果本方法的相对标准偏差(n=6)在0.92%～3.2%之间，平均回收率在86.4%～99.1%之间，检出限在0.005～0.008 mg/kg之间。结论本方法灵敏度高，分离效果好，结果稳定可靠，适用于熟肉制品中日落黄、胭脂红和诱惑红的同时测定。
목적：건립대숙육제품중일락황、연지홍화유혹홍삼충합성색소동시측정적방법。방법양품채용무수을순-안수-수(7：2：1)제취，가정기완거제지방，담취후정용，용고효액상색보법측정。경Symmetry Shield RP18주분리，갑순-0.02 mol/L을산안용액제도세탈，검측파장위508 nm。결과본방법적상대표준편차(n=6)재0.92%～3.2%지간，평균회수솔재86.4%～99.1%지간，검출한재0.005～0.008 mg/kg지간。결론본방법령민도고，분리효과호，결과은정가고，괄용우숙육제품중일락황、연지홍화유혹홍적동시측정。
Objective To establish a method of simultaneous determination of sunset yellow, carmine and allura red up to three synthetic pigments in cooked meat products.Methods The samples were extracted by the anhydrousethanol-ammonia-water solution (7:2:1), the fat was eliminated by hexane, then blown by nitrogen, analyzed by high performance liquid chromatography. The samples were separated by SymmetryShield RP18 column, by gradient elution using a mobile phase made up of methanol and 0.02 mol/L amm-onium acetate, and detected at 508 nm.Results The relative standard derivations (n=6)were between 0.92%~3.2%, the recoveries were between 86.4%~99.1%, the detection limits were between 0.005~0.008 mg/kg.Conclusion This method is sensitive, efficient, reliable and suitable for the determination of synthetic pigments in cooked meat products.