国际儿科学杂志
國際兒科學雜誌
국제인과학잡지
INTERNATIONAL JOURNAL OF PEDIATRICS
2014年
6期
644-648
,共5页
足细胞%阿霉素%哺乳动物雷帕霉素靶蛋白%小鼠
足細胞%阿黴素%哺乳動物雷帕黴素靶蛋白%小鼠
족세포%아매소%포유동물뢰파매소파단백%소서
Podosytes%Adriamycin%mammalian target of rapamycin%Mouse
目的 探讨哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)复合体在阿霉素损伤足细胞中的作用.方法 用1 μmol/L的阿霉素(adriamycin,ADM)诱导小鼠足细胞24 h,检测足细胞活力、足细胞凋亡比例、足细胞损伤标志物结蛋白(Desmin)和mTOR相关分子的表达,分析mTOR复合体在ADM损伤足细胞中的作用.结果 (1)1μmol/L的ADM诱导足细胞24h,可使足细胞损伤.与对照组比较,足细胞活性下降(t =28.68,P＜0.001),足细胞早期凋亡比例增加(t=10.24,P=0.09),晚期凋亡比例也增加(t =-4.26,P=0.013);损伤标志分子Desmin表达增多(t=6.16 P＜0.001);(2) ADM诱导组足细胞mTOR蛋白表达下降,与对照组比较,mTOR和磷酸化mTOR表达均下降(t=3.57,P=0.023),mTOR下游分子S6K1和4EBP1 mRNA表达下降(t=18.28,P＜0.001).结论 ADM可诱导足细胞损伤,ADM损伤足细胞的机制可能与mTOR蛋白减少、活性下降有关.
目的 探討哺乳動物雷帕黴素靶蛋白(mammalian target of rapamycin,mTOR)複閤體在阿黴素損傷足細胞中的作用.方法 用1 μmol/L的阿黴素(adriamycin,ADM)誘導小鼠足細胞24 h,檢測足細胞活力、足細胞凋亡比例、足細胞損傷標誌物結蛋白(Desmin)和mTOR相關分子的錶達,分析mTOR複閤體在ADM損傷足細胞中的作用.結果 (1)1μmol/L的ADM誘導足細胞24h,可使足細胞損傷.與對照組比較,足細胞活性下降(t =28.68,P＜0.001),足細胞早期凋亡比例增加(t=10.24,P=0.09),晚期凋亡比例也增加(t =-4.26,P=0.013);損傷標誌分子Desmin錶達增多(t=6.16 P＜0.001);(2) ADM誘導組足細胞mTOR蛋白錶達下降,與對照組比較,mTOR和燐痠化mTOR錶達均下降(t=3.57,P=0.023),mTOR下遊分子S6K1和4EBP1 mRNA錶達下降(t=18.28,P＜0.001).結論 ADM可誘導足細胞損傷,ADM損傷足細胞的機製可能與mTOR蛋白減少、活性下降有關.
목적 탐토포유동물뢰파매소파단백(mammalian target of rapamycin,mTOR)복합체재아매소손상족세포중적작용.방법 용1 μmol/L적아매소(adriamycin,ADM)유도소서족세포24 h,검측족세포활력、족세포조망비례、족세포손상표지물결단백(Desmin)화mTOR상관분자적표체,분석mTOR복합체재ADM손상족세포중적작용.결과 (1)1μmol/L적ADM유도족세포24h,가사족세포손상.여대조조비교,족세포활성하강(t =28.68,P＜0.001),족세포조기조망비례증가(t=10.24,P=0.09),만기조망비례야증가(t =-4.26,P=0.013);손상표지분자Desmin표체증다(t=6.16 P＜0.001);(2) ADM유도조족세포mTOR단백표체하강,여대조조비교,mTOR화린산화mTOR표체균하강(t=3.57,P=0.023),mTOR하유분자S6K1화4EBP1 mRNA표체하강(t=18.28,P＜0.001).결론 ADM가유도족세포손상,ADM손상족세포적궤제가능여mTOR단백감소、활성하강유관.
Objective To explore the role of mammalian target of rapamycin (mTOR) complex in the adriamycin induced the podecytes.Methods 1 μmol/L adriamycin (ADM) was used to induce mice podocytes for 24 hours and detected the apoptosis ratio,podosytes damage markers (Desmin) and the related expression of mTOR,then the role of mTOR was analysized in ADM induced podosytes.Results 1 umol/L ADM induced mice podosytes for 24 hours can make the podocytes damage.Compared with control group,podosytes activity declined(t =28.68,P ＜ 0.001),apoptosis rate increased both early (t =10.24,P =10.24) and late (t =4.26,P =4.26),and Desmin expression increased(t =6.16,P ＜0.001).mTOR expression decreased in ADM group.Compared with control group,both mTOR and phosphorylation mTOR expression decreased (t =3.57,P =3.57).mTOR downstream target molecular S6K1 and 4ebpl mRNA expression decreased(t =18.28,P ＜ 0.001).Conclusion ADM can make podosytes damage and the mechanism of ADM induced podosytes damage may be related to the decreasing mTOR and the low activity of mTOR.
