CAJ | 학술논문

目的：比较胶原酶的不同消化时间，观察所获得的间充质干细胞的数量，确定最为理想的消化时间。方法：分别采用3h、5h、7h的消化时间分离脐带间充质干细胞；通过传代进行纯化和扩增培养，绘制生长曲线；用流式细胞仪检测其表面标志。结果：分离出的间充质干细胞贴壁后为梭形，呈平行排列生长或漩涡状生长，并且消化时间为5h时收获到的细胞数量最多；流式细胞仪检测结果显示，获得的间充质干细胞均表达CD73、CD105、HLA－ABC，不表达CD34、CD45、HLA－DR。结论：胶原酶消化法从人脐带中分离培养的细胞具有间充质干细胞的生物学特性，并且消化5h收获得的间充质干细胞数量最多，生长状况最好。
목적：비교효원매적불동소화시간，관찰소획득적간충질간세포적수량，학정최위이상적소화시간。방법：분별채용3h、5h、7h적소화시간분리제대간충질간세포；통과전대진행순화화확증배양，회제생장곡선；용류식세포의검측기표면표지。결과：분리출적간충질간세포첩벽후위사형，정평행배렬생장혹선와상생장，병차소화시간위5h시수획도적세포수량최다；류식세포의검측결과현시，획득적간충질간세포균표체CD73、CD105、HLA－ABC，불표체CD34、CD45、HLA－DR。결론：효원매소화법종인제대중분리배양적세포구유간충질간세포적생물학특성，병차소화5h수획득적간충질간세포수량최다，생장상황최호。
Objective Comparing the quantity of MSCs acquired from umbilical cord that digested by collagenase II in different hours,ascertain an ideal digesting time and culture and appraise the cells acquired.Methods MSCs were isolated from human umbilical cord through collagenase digestion respectively in 3 h,5 h,7 h,then passaged to purify and culture,drew the growth curve.Their surface markers and cells cycle were detected by flow cytometry.Results MSCs isolated from umbilical cord ad-hered with a spindle-shape,they parallel along their longitudinal axis or grew in whirl manner.After 5 hours digestion,we got the biggest quantity with best growth status,and successfully built the method for MSCs culture and increasing.Detected by flow cytometry,all of adherent cells express CD73、CD105、HLA-ABC,none of them express CD34、CD45、HLA-DR.Con-clusion Cells isolated from umbilical cord by digestion have the biological characteristics of MSCs.And 5 hours is the best time for digestion to acquire the most and best MSCs.