CAJ | 학술논문

目的：观察麝香乌龙丸对糖尿病（ DM）兔骨折愈合的影响，并探讨其机制。方法将36只大白兔随机分为A、B、C组各12只。 B、C组建立DM模型，3组均造成右胫腓骨骨折。 C组将麝香乌龙丸以1．0 g／kg剂量溶于5 mL生理盐水中灌胃，A、B组给予等量生理盐水灌胃。3组分别于给药后第1、2、4周各取4只大白兔，麻醉后采用Norland．XR 36双能X线骨密度测量仪检测骨折端骨密度，取骨痂行HE染色观察形态学变化，采用Western blot法检测骨痂神经生长因子（ NGF）相对表达。结果 B组局部骨密度在骨折愈合过程中出现下降，给药1～2周时略微下降，第4周下降明显，其下降趋势较A、C组更加明显。 B组各时点骨密度均低于A、C组，P均＜0．05。3组用药后第1周的骨痂形态学均无明显变化；用药第2、4周与B组比较，A组可见大量较成熟软骨存在，形成软骨岛，骨小梁生成并发育成熟；C组未见软骨岛存在，但软骨性骨痂加速向骨性骨痂转化。 B、C组用药第2、4周的骨痂NGF相对表达均较第1周升高，B组各时点骨痂NGF相对表达均低于A、C组，P均＜0．05；A、C组各时点骨痂NGF相对表达比较，P均＞0．05。结论麝香乌龙丸可以加速DM兔骨折愈合，可能与上调骨痂NGF的表达有关。
목적：관찰사향오룡환대당뇨병（ DM）토골절유합적영향，병탐토기궤제。방법장36지대백토수궤분위A、B、C조각12지。 B、C조건립DM모형，3조균조성우경비골골절。 C조장사향오룡환이1．0 g／kg제량용우5 mL생리염수중관위，A、B조급여등량생리염수관위。3조분별우급약후제1、2、4주각취4지대백토，마취후채용Norland．XR 36쌍능X선골밀도측량의검측골절단골밀도，취골가행HE염색관찰형태학변화，채용Western blot법검측골가신경생장인자（ NGF）상대표체。결과 B조국부골밀도재골절유합과정중출현하강，급약1～2주시략미하강，제4주하강명현，기하강추세교A、C조경가명현。 B조각시점골밀도균저우A、C조，P균＜0．05。3조용약후제1주적골가형태학균무명현변화；용약제2、4주여B조비교，A조가견대량교성숙연골존재，형성연골도，골소량생성병발육성숙；C조미견연골도존재，단연골성골가가속향골성골가전화。 B、C조용약제2、4주적골가NGF상대표체균교제1주승고，B조각시점골가NGF상대표체균저우A、C조，P균＜0．05；A、C조각시점골가NGF상대표체비교，P균＞0．05。결론사향오룡환가이가속DM토골절유합，가능여상조골가NGF적표체유관。
Objective To observe the effects and mechanisms of Mask Dark Dragon Pelle on diabetes mellitus ( DM) rabbits fracture healing.Methods A totle of 36 rabbits were divided into A, B, C groups, 12 rabbits in each group.B and C groups were DM models , A group was normal control .Then fracture of the right tibia and fibula were builted in three groups.The rabbits in C group were received intragastric administration of 1.0 g/kg Mask Dark Dragon Pelle +5 mL nor-mal saline, the equal volume of normal saline were given in A and B groups .At 1, 2, 4 weeks after treatment, four rabbits in each group were taken to study .The bone mineral density ( BMD ) was detected by dual-energy X-ray absorptiometry ( DEXA);and the callus were collected for HE staining to detect pathological changes ;the level of NGF in callus was test-ed by Western blot .Results BMD in B group showed an downward trend during the process of fracture healing , the BMD in B group decreased than A and C groups at 1, 2, 4 weeks (P均<0.05).Among three groups, group C displayed the best callus formation in pathological changes , and fracture healing was significantly better than that in the other two groups . At the same time, the expression of NGF was significantly higher in group C than that in group B ( P<0.05).Conclu-sions The expression of NGF decreased in callus of DM rabbits .Mask Dark Dragon Pelle accelerates the DM fracture healing, its mechanism maybe related to NGF expression up-regulation.