色谱
色譜
색보
CHINESE JOURNAL OF CHROMATOGRAPHY
2015年
1期
17-21
,共5页
超高效液相色谱-串联质谱%染料%染发剂
超高效液相色譜-串聯質譜%染料%染髮劑
초고효액상색보-천련질보%염료%염발제
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)%dyes%hair dyes
建立了同时测定染发剂中4，4′-二氨基二苯胺硫酸盐和2，4-二氨基苯酚硫酸盐等6种染料的超高效液相色谱-串联质谱（UPLC-MS／MS）分析方法。以水为提取剂进行超声提取，用 Waters BEH-C18柱（100 mm×2.1 mm，1.7μm）分离，以10 mmol／L的乙酸铵和乙腈为流动相进行梯度洗脱。在电喷雾正离子模式下，采用多反应监测（MRM）模式进行定性和定量分析。6种染料在相应的质量浓度范围内线性关系良好，线性相关系数（R2）均大于0.99，检出限为0.26~4.6 mg／kg，回收率为83.0％~92.2％，相对标准偏差为5.4％~11.2％。方法精密度、准确度、回收率和基质效应均符合染发剂样品测定的要求，用于实际样品测定，结果满意。本方法准确、简便、快速，适用于染发剂中6种染料的分析测定。
建立瞭同時測定染髮劑中4，4′-二氨基二苯胺硫痠鹽和2，4-二氨基苯酚硫痠鹽等6種染料的超高效液相色譜-串聯質譜（UPLC-MS／MS）分析方法。以水為提取劑進行超聲提取，用 Waters BEH-C18柱（100 mm×2.1 mm，1.7μm）分離，以10 mmol／L的乙痠銨和乙腈為流動相進行梯度洗脫。在電噴霧正離子模式下，採用多反應鑑測（MRM）模式進行定性和定量分析。6種染料在相應的質量濃度範圍內線性關繫良好，線性相關繫數（R2）均大于0.99，檢齣限為0.26~4.6 mg／kg，迴收率為83.0％~92.2％，相對標準偏差為5.4％~11.2％。方法精密度、準確度、迴收率和基質效應均符閤染髮劑樣品測定的要求，用于實際樣品測定，結果滿意。本方法準確、簡便、快速，適用于染髮劑中6種染料的分析測定。
건립료동시측정염발제중4，4′-이안기이분알류산염화2，4-이안기분분류산염등6충염료적초고효액상색보-천련질보（UPLC-MS／MS）분석방법。이수위제취제진행초성제취，용 Waters BEH-C18주（100 mm×2.1 mm，1.7μm）분리，이10 mmol／L적을산안화을정위류동상진행제도세탈。재전분무정리자모식하，채용다반응감측（MRM）모식진행정성화정량분석。6충염료재상응적질량농도범위내선성관계량호，선성상관계수（R2）균대우0.99，검출한위0.26~4.6 mg／kg，회수솔위83.0％~92.2％，상대표준편차위5.4％~11.2％。방법정밀도、준학도、회수솔화기질효응균부합염발제양품측정적요구，용우실제양품측정，결과만의。본방법준학、간편、쾌속，괄용우염발제중6충염료적분석측정。
A sensitive method was developed for the simultaneous determination of six compo-nents which included 4,4′-diaminodiphenylamine sulfate hydrate and 2,4-diaminophenol sul-fate,etc. in hair dyes by ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS). After extracted by water through ultrasonic extraction,the samples were ana-lyzed by UPLC-MS/MS. The separation was performed on a Waters BEH-C 18 column( 100 mm × 2. 1 mm,1. 7 μm)with gradient elution of 10 mmol/L ammonium acetate and acetonitrile. The electrospray ionization( ESI)source in positive ion mode was used for the analysis of the six components in the multiple reaction monitoring( MRM)mode. The results showed good linear relationships with all the correlation coefficients(R2)more than 0. 99. The limits of detection (LODs,S/N=3)for the six components were in the range of 0. 26-4. 6 mg/kg. The average recoveries of the six components in the spiked samples were in the range of 83. 0%-92. 2% with the relative standard deviations( RSDs,n=6)of 5. 4%-11. 2%. The precision,accuracy,mean recoveries and the matrix effects satisfied the requirements of cosmetic sample measurement. The proposed method has been applied to the determination of six dyes in actual samples. This method is simple,accurate and effective for the simultaneous determination of the six compo-nents in hair dyes.
