CAJ | 학술논문

建立了罐头食品中尿素残留检测的柱前衍生-高效液相色谱-荧光检测方法。取5.0 g样品，经20 mL 1％（ v／v）乙酸溶液提取、定容，离心后取上清液过滤，吸取0.5 mL 提取液用呫吨醇进行衍生，采用高效液相色谱-荧光检测器进行测定。尿素在0.1~500 mg／L内线性良好，相关系数大于0.9995。实验表明，在5种罐头食品中各添加0.001~30 g／kg 尿素，其平均回收率为80.2％~109.7％，相对标准偏差（ n=6）为2.05％~6.53％，检出限为0.5 mg／kg，定量限为1.0 mg／kg。利用本研究建立的方法对168个实际样品进行测定，在3个肉类罐头样品中检出尿素，含量分别为10.6、62.1和2.6 mg／kg。方法稳定、可靠、操作简单，适用于罐头食品中尿素的检测。
건립료관두식품중뇨소잔류검측적주전연생-고효액상색보-형광검측방법。취5.0 g양품，경20 mL 1％（ v／v）을산용액제취、정용，리심후취상청액과려，흡취0.5 mL 제취액용첩둔순진행연생，채용고효액상색보-형광검측기진행측정。뇨소재0.1~500 mg／L내선성량호，상관계수대우0.9995。실험표명，재5충관두식품중각첨가0.001~30 g／kg 뇨소，기평균회수솔위80.2％~109.7％，상대표준편차（ n=6）위2.05％~6.53％，검출한위0.5 mg／kg，정량한위1.0 mg／kg。이용본연구건립적방법대168개실제양품진행측정，재3개육류관두양품중검출뇨소，함량분별위10.6、62.1화2.6 mg／kg。방법은정、가고、조작간단，괄용우관두식품중뇨소적검측。
A method for the determination of urea residue in canned foods by high performance liquid chromatography-fluorescence detection( HPLC-FLD)coupled with precolumn derivatiza-tion was established. The sample(5. 0 g),including canned edible fungi,fruit,vegetable,fish, and meat was extracted with 20 mL 1%( v/v)acetic acid solution. The extract was centrifuged, filtrated,and then derivatized with xanthydrol. The analysis was completed with HPLC-FLD. A good linear relationship was obtained in the range of 0. 1-500 mg/L with the correlation coeffi-cients more than 0. 999 5. The average recoveries of urea spiked at 0. 001-30 g/kg levels in five kinds of canned foods ranged from 80. 2% to 109. 7% with the RSDs of 2. 05%-6. 53%. The limit of detection(LOD)was 0. 5 mg/kg,and the limit of quantitation(LOQ)was 1. 0 mg/kg. The proposed procedure was then applied to the analysis of 168 real samples collected from Xia-men,Fujian Province,China. The existence of urea was found in three pork cans with contents of 10. 6,62. 1 and 2. 6 mg/kg,respectively. The method is stable,reliable,simple and suitable for the determination of urea in canned foods,and has great potential for routine analysis in foodstuffs.