CAJ | 학술논문

建立了一种高效液相色谱法快速测定水基胶黏剂中3种异噻唑啉酮类杀菌剂（2-甲基-4-异噻唑啉-3-酮（MI）、5-氯-2-甲基-4-异噻唑啉-3-酮（CMI）和1，2-苯并异噻啉-3-酮（BIT））的分析方法。样品经甲醇-水（1：1，v／v）溶液振荡提取、离心、过滤后，采用高效液相色谱-二极管阵列检测器检测，C18色谱柱分离，流动相为甲醇-水，梯度洗脱。对前处理条件（包括萃取溶剂、提取方式、稀释倍数、提取时间）进行了优化。在优化实验条件下，样品中的异噻唑啉酮在0.25~10.0 mg／L范围内呈良好的线性关系（ r2≥0.9992），加标回收率在92％~103％之间，相对标准偏差不高于4％，检出限为0.43~1.14 mg／kg，定量限为1.44~3.81 mg／kg。结果表明该方法能达到定量检测的目的。将该方法应用于实际样品的检测，结果可靠。
건립료일충고효액상색보법쾌속측정수기효점제중3충이새서람동류살균제（2-갑기-4-이새서람-3-동（MI）、5-록-2-갑기-4-이새서람-3-동（CMI）화1，2-분병이새람-3-동（BIT））적분석방법。양품경갑순-수（1：1，v／v）용액진탕제취、리심、과려후，채용고효액상색보-이겁관진렬검측기검측，C18색보주분리，류동상위갑순-수，제도세탈。대전처리조건（포괄췌취용제、제취방식、희석배수、제취시간）진행료우화。재우화실험조건하，양품중적이새서람동재0.25~10.0 mg／L범위내정량호적선성관계（ r2≥0.9992），가표회수솔재92％~103％지간，상대표준편차불고우4％，검출한위0.43~1.14 mg／kg，정량한위1.44~3.81 mg／kg。결과표명해방법능체도정량검측적목적。장해방법응용우실제양품적검측，결과가고。
A rapid high performance liquid chromatography( HPLC)method was developed for the quantitative analysis of three isothiazolinone biocides( 2-methyl-4-isothiazolin-3-one( MI), 5-chloro-2-methyl-4-isothiazolin-3-one ( CMI ) and 1,2-benzylisothiazolin-3-one ( BIT )) in water-borne adhesives. The sample was extracted with methanol-water(1:1,v/v),and puri-fied by centrifugation and filtration. The isothiazolones were separated on a C18 column with methanol-water as mobile phases under gradient elution and detected with a diode array detector( DAD). The pretreatment factors such as extraction solvent,extraction method,dilu-tion ratio,extraction time were optimized. Under the optimized conditions,the targets had good linearities( r2≥0. 999 2)in the range of 0. 25-10. 0 mg/L. The recoveries were between 92% and 103% with the relative standard deviations not more than 4%. The limits of detection ( LODs)were between 0. 43 mg/kg and 1. 14 mg/kg. The limits of quantification( LOQs)were between 1. 44 mg/kg and 3. 81 mg/kg. The results showed that the method can achieve the pur-pose of quantitative detection. The analyses of real samples verified the reliability of this method.