中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2015年
4期
1-2
,共2页
冠心病%糖尿病%糖基化终产物%toll样受体4
冠心病%糖尿病%糖基化終產物%toll樣受體4
관심병%당뇨병%당기화종산물%toll양수체4
Coronary heart disease%Diabetes mellitus%Advanced glycation end products%Toll-like receptor 4
目的：探讨分析冠心病(CHD)合并糖尿病(DM)患者糖基化终产物(AGEs)与单核细胞toll样受体4(TLR4)的关系。方法CHD+DM患者58例(CHD+DM组), CHD患者60例(CHD组),健康对照组50例。采用酶联免疫吸附测定(ELISA)法检测各组的AGEs,用流式细胞仪检测外周血单核细胞TLR4mRNA相对表达量及TLR4阳性单核细胞比率。结果与健康对照组比较, CHD+DM组及CHD组AGEs及外周血单核细胞TLR4mRNA相对表达量、TLR4阳性单核细胞比率明显增高(P<0.05)；CHD+DM组与CHD组比较, CHD+DM组AGEs及外周血单核细胞TLR4mRNA相对表达量、TLR4阳性单核细胞比率增高(P<0.05)；相关性分析显示升高的AGEs与升高的TLR4mRNA相对表达量及TLR4阳性单核细胞比率呈正相关。结论 CHD+DM患者AGEs及TLR4表达高于CHD患者；CHD患者AGEs及TLR4表达高于健康对照组。AGEs与TLR4的表达呈正相关。
目的：探討分析冠心病(CHD)閤併糖尿病(DM)患者糖基化終產物(AGEs)與單覈細胞toll樣受體4(TLR4)的關繫。方法CHD+DM患者58例(CHD+DM組), CHD患者60例(CHD組),健康對照組50例。採用酶聯免疫吸附測定(ELISA)法檢測各組的AGEs,用流式細胞儀檢測外週血單覈細胞TLR4mRNA相對錶達量及TLR4暘性單覈細胞比率。結果與健康對照組比較, CHD+DM組及CHD組AGEs及外週血單覈細胞TLR4mRNA相對錶達量、TLR4暘性單覈細胞比率明顯增高(P<0.05)；CHD+DM組與CHD組比較, CHD+DM組AGEs及外週血單覈細胞TLR4mRNA相對錶達量、TLR4暘性單覈細胞比率增高(P<0.05)；相關性分析顯示升高的AGEs與升高的TLR4mRNA相對錶達量及TLR4暘性單覈細胞比率呈正相關。結論 CHD+DM患者AGEs及TLR4錶達高于CHD患者；CHD患者AGEs及TLR4錶達高于健康對照組。AGEs與TLR4的錶達呈正相關。
목적：탐토분석관심병(CHD)합병당뇨병(DM)환자당기화종산물(AGEs)여단핵세포toll양수체4(TLR4)적관계。방법CHD+DM환자58례(CHD+DM조), CHD환자60례(CHD조),건강대조조50례。채용매련면역흡부측정(ELISA)법검측각조적AGEs,용류식세포의검측외주혈단핵세포TLR4mRNA상대표체량급TLR4양성단핵세포비솔。결과여건강대조조비교, CHD+DM조급CHD조AGEs급외주혈단핵세포TLR4mRNA상대표체량、TLR4양성단핵세포비솔명현증고(P<0.05)；CHD+DM조여CHD조비교, CHD+DM조AGEs급외주혈단핵세포TLR4mRNA상대표체량、TLR4양성단핵세포비솔증고(P<0.05)；상관성분석현시승고적AGEs여승고적TLR4mRNA상대표체량급TLR4양성단핵세포비솔정정상관。결론 CHD+DM환자AGEs급TLR4표체고우CHD환자；CHD환자AGEs급TLR4표체고우건강대조조。AGEs여TLR4적표체정정상관。
Objective To investigate the relationship between advanced glycation end products (AGEs) and monocyte Toll-like receptor 4 (TLR4) in patients with coronary heart disease (CHD) combined with diabetes mellitus (DM).Methods There were 58 patients with CHD+DM (CHD+DM group), 60 patients with CHD only (CHD group), and 50 patients in healthy control group. Enzyme-linked immuno sorbent assay (ELISA) was applied to detect AGEs in all the groups, and flow cytometry was used to detect relative expression of peripheral blood monocytes TLR4mRNA and ratio of TLR4 positive monocytes.Results Compared with the healthy control group, CHD+DM group and CHD group all had remarkably increased AGEs, relative expression of peripheral blood monocytes TLR4mRNA, and ratio of TLR4 positive monocytes (P<0.05). Between the CHD+DM group and CHD group, the former had higher increased levels of AGEs, relative expression of peripheral blood monocytes TLR4mRNA, and ratio of TLR4 positive monocytes (P<0.05). The results of correlated analysis showed that there was positive correlation between increased AGEs, increased relative expression of TLR4mRNA, and ratio of TLR4 positive monocytes. Conclusion The expressions of AGEs and TLR4 of CHD+DM patients are higher than CHD patients, and those of CHD patients are also higher than healthy control group. AGEs and expression of TLR4 are positively correlated.
