CAJ | 학술논문

目的：观察降压通络方对自发性高血压肾损害大鼠肾脏血管紧张素II （ angiotensin II， AngII）及肾功能的影响，探讨该方对高血压肾损害大鼠肾脏的保护机制。方法采用16周龄自发性高血压大鼠（Spontaneously Hypertension Rat ， SHR）为研究对象，将其随机分为模型组、缬沙坦组、降压通络方高、中、低剂量组、并设正常血压大鼠（ Wistar Kyoto， WKY）为空白对照，分别灌胃给药。于给药后4周和8周分别测定大鼠尾动脉压力、血肌酐、尿素氮及肾脏AngII蛋白含量，并进行大鼠肾脏病理组织学检查。结果给药4、8周后，与模型组比较，各治疗组大鼠血压、肾脏AngII含量明显降低（P＜0．01，P＜0．05），但给药4周各组之间比较无统计学意义，给药8周缬沙坦及降压通络方高、中剂量组均明显低于低剂量组（ P＜0．05）；缬沙坦及降压通络方能显著降低模型组大鼠血肌酐、尿素氮（ P＜0．01，P＜0．05），改善肾小动脉及肾小球硬化，促进肾组织结构的恢复。结论降压通络方能降低高血压肾损伤大鼠肾脏AngII含量，从而有效降低大鼠血压，改善肾脏病理损害，保护肾功能。
목적：관찰강압통락방대자발성고혈압신손해대서신장혈관긴장소II （ angiotensin II， AngII）급신공능적영향，탐토해방대고혈압신손해대서신장적보호궤제。방법채용16주령자발성고혈압대서（Spontaneously Hypertension Rat ， SHR）위연구대상，장기수궤분위모형조、힐사탄조、강압통락방고、중、저제량조、병설정상혈압대서（ Wistar Kyoto， WKY）위공백대조，분별관위급약。우급약후4주화8주분별측정대서미동맥압력、혈기항、뇨소담급신장AngII단백함량，병진행대서신장병리조직학검사。결과급약4、8주후，여모형조비교，각치료조대서혈압、신장AngII함량명현강저（P＜0．01，P＜0．05），단급약4주각조지간비교무통계학의의，급약8주힐사탄급강압통락방고、중제량조균명현저우저제량조（ P＜0．05）；힐사탄급강압통락방능현저강저모형조대서혈기항、뇨소담（ P＜0．01，P＜0．05），개선신소동맥급신소구경화，촉진신조직결구적회복。결론강압통락방능강저고혈압신손상대서신장AngII함량，종이유효강저대서혈압，개선신장병리손해，보호신공능。
Objective o observe the effects of Jiangya Tongluo Formula on the content of AngII in kidney and the renal function in the model of hypertension nephrosclerosis .Methods Sixteen-week-old Spontaneously Hypertension rats were used as research subjects and then the rats were randomly divided in -to 5 groups:a model group , a Valsartan group , 3 Jiangya Tongluo groups and Wistar Kyoto rats were used as control group.All the rats were gavaged respectively .After 4 weeks and 8 weeks, the tail arterial pres-sure of the rats, Serum creatinine , Urea nitrogen and the dose of AngII in kidney were detected , At the same time, the histopathologic change in kidney tissue was also detected .Results After 4 weeks and 8 weeks gavage , compared to model group .The tail arterial pressure and the level of Ang II in kidney was decreased in Jiangya Tongluo groups and Valsartan group (P<0.01,P<0.05).But after 4 weeks gavage, there was no significant difference in therapeutic groups .After 8 weeks gavage , the date in Valsartan and Jiangya Tongluo high and medium dose groups were lower significantly than that of low dose group ( P<0.05).Valsartan and Jiangyatongluo degraded the concentration of SCr , BUN in blood-serum significantly (P<0.01,P<0.05), improved the renal arteries and glomerular sclerosis , promoted the recovery of kid-ney tissue .Conclusion Jiangya Tongluo Formula could reduce the level of Ang II in kidney ,decrease blood pressure , ameliorate renal pathologicallesion and protect renal function .