CAJ | 학술논문

目的：观察通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响。方法采用高脂饲料+小剂量链脲佐菌素建立2型糖尿病大鼠模型，随机分为空白组10只、模型组10只、缬沙坦组10只（10mg／kg／d）和通心络组10只（0．4mg／kg／d）；治疗8周后，比较各组大鼠的空腹血糖、尿白蛋白排泄率、内生肌酐清除率及肾重指数，采用酶联免疫吸附试验测定血浆血管内皮生长因子含量，western blot及RT－PCR测定肾组织中血管内皮生长因子及其受体Flt1的表达。结果模型组大鼠空腹血糖、尿白蛋白排泄率、内生肌酐清除率、肾重指数、血浆血管内皮生长因子含量、肾组织血管内皮生长因子及Flt1表达较空白组有不同程度的升高（P＜0．05），通心络组除空腹血糖外各指标水平较模型组均显著降低（ P＜0．05）。结论通心络可以有效改善早期糖尿病肾病大鼠的高滤过状态，抑制尿蛋白的排出，其作用机制与抑制血管内皮生长因子及其受体Flt1的过度表达有关。
목적：관찰통심락대조기당뇨병신병대서혈관내피생장인자표체적영향。방법채용고지사료+소제량련뇨좌균소건립2형당뇨병대서모형，수궤분위공백조10지、모형조10지、힐사탄조10지（10mg／kg／d）화통심락조10지（0．4mg／kg／d）；치료8주후，비교각조대서적공복혈당、뇨백단백배설솔、내생기항청제솔급신중지수，채용매련면역흡부시험측정혈장혈관내피생장인자함량，western blot급RT－PCR측정신조직중혈관내피생장인자급기수체Flt1적표체。결과모형조대서공복혈당、뇨백단백배설솔、내생기항청제솔、신중지수、혈장혈관내피생장인자함량、신조직혈관내피생장인자급Flt1표체교공백조유불동정도적승고（P＜0．05），통심락조제공복혈당외각지표수평교모형조균현저강저（ P＜0．05）。결론통심락가이유효개선조기당뇨병신병대서적고려과상태，억제뇨단백적배출，기작용궤제여억제혈관내피생장인자급기수체Flt1적과도표체유관。
Objective To observe the effect of Tongxinluo on vascular endothelial growth factor ( VEGF) expression in rats with early stage of diabetic nephropathy .Methods The rat model of type 2 di-abetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet for 4 weeks, 40 rats were randomly divided into 4 groups-normal group, diabetic group, valsartan treated group(10mg/kg/d) and Tongxinluo treated group (0.4mg/kg/d);After 8 weeks, the level of urinary albumin excretion rate (UAER), creatinine clearance rate (Ccr),kidney weight/body weight ratio (KW/BW) and plasma VEGF was measured and the renal expression of VEGF and Flt 1 was determined by western blot and real-time PCR.Results The level of UAER, Ccr, KW/BW, VEGF an Flt1 expression in Tongxinluo treated group was lower than it in diabetic group (P<0.05),while FBG had no significant difference .Conclusion Tongxinluo can improve glomerular hyperfiltration and albuminuria excretion in rats with early stage of di -abetic nephropathy;The mechanism could be related to inhibition of VEGF and Flt 1 expression .