临床合理用药杂志
臨床閤理用藥雜誌
림상합리용약잡지
CHINESE JOURNAL OF CLINICAL RATIONAL DRUG USE
2014年
34期
26-27
,共2页
消骨痛汤%骨关节炎%TGFβ1%BMP2
消骨痛湯%骨關節炎%TGFβ1%BMP2
소골통탕%골관절염%TGFβ1%BMP2
Xiaogutong decoction%Osteoarthritis%TGFβ1%BMP2
目的：观察中药方剂消骨痛汤对骨关节炎（OA）大鼠模型中软骨和滑膜中转化生长因子β1（TGFβ1）和骨形成蛋白2（BMP2）的表达。方法将40只Wistar大鼠随机分为4组，每组10只。正常对照组不予干预，其余3组采用膝关节腔内注射木瓜蛋白酶的方法建立骨关节炎大鼠模型，造模2周后开始给药，分别给予生理盐水（模型组）、双氯芬酸钠（西药组）和中药消骨痛汤（中药组），灌胃8周后取软骨及滑膜标本。用免疫组化方法检测TGFβ1和BMP2在软骨和滑膜中的蛋白表达。结果模型组和西药组软骨、滑膜中TGFβ1、BMP2水平低于中药组和正常组，差异均有统计学意义（P＜0．05）。中药组和正常组TGFβ1和BMP2水平差异无统计学意义（P＞0．05）。结论消骨痛汤能上调OA大鼠软骨和滑膜中TGFβ1和BMP2的蛋白表达。
目的：觀察中藥方劑消骨痛湯對骨關節炎（OA）大鼠模型中軟骨和滑膜中轉化生長因子β1（TGFβ1）和骨形成蛋白2（BMP2）的錶達。方法將40隻Wistar大鼠隨機分為4組，每組10隻。正常對照組不予榦預，其餘3組採用膝關節腔內註射木瓜蛋白酶的方法建立骨關節炎大鼠模型，造模2週後開始給藥，分彆給予生理鹽水（模型組）、雙氯芬痠鈉（西藥組）和中藥消骨痛湯（中藥組），灌胃8週後取軟骨及滑膜標本。用免疫組化方法檢測TGFβ1和BMP2在軟骨和滑膜中的蛋白錶達。結果模型組和西藥組軟骨、滑膜中TGFβ1、BMP2水平低于中藥組和正常組，差異均有統計學意義（P＜0．05）。中藥組和正常組TGFβ1和BMP2水平差異無統計學意義（P＞0．05）。結論消骨痛湯能上調OA大鼠軟骨和滑膜中TGFβ1和BMP2的蛋白錶達。
목적：관찰중약방제소골통탕대골관절염（OA）대서모형중연골화활막중전화생장인자β1（TGFβ1）화골형성단백2（BMP2）적표체。방법장40지Wistar대서수궤분위4조，매조10지。정상대조조불여간예，기여3조채용슬관절강내주사목과단백매적방법건립골관절염대서모형，조모2주후개시급약，분별급여생리염수（모형조）、쌍록분산납（서약조）화중약소골통탕（중약조），관위8주후취연골급활막표본。용면역조화방법검측TGFβ1화BMP2재연골화활막중적단백표체。결과모형조화서약조연골、활막중TGFβ1、BMP2수평저우중약조화정상조，차이균유통계학의의（P＜0．05）。중약조화정상조TGFβ1화BMP2수평차이무통계학의의（P＞0．05）。결론소골통탕능상조OA대서연골화활막중TGFβ1화BMP2적단백표체。
Objective To observe the influence of Xiaogutong decoction in TGFβ1 and BMP2 of rats with osteoarthri-tis.Methods 40 cases rats were randomly divided into 4 groups,each of 10 cases.Normal control group with no intervention. The remaining 3 groups to establish osteoarthritis rat model.Dosing in 2 weeks after modeling.Respectively treated by normal saline,diclofenac and Xiaogutong decoction.After 8 weeks,taken the cartilage and synovial samples.TGFβ1 and BMP2 were de-tected by immunohistochemistry.Compared the level of TGFβ1 and BMP2 of 4 groups.Results The level of TGFβ1 and BMP2 in model group and western medicine group were lower than that of normal group and chinese medicine group,the difference was statistically significant(P<0.05).The level of TGFβ1 and BMP2 in normal group and chinese medicine group has no sta-tistically significant(P>0.05).Conclusion Xiaogutong decoction can improve the expression of the TGFβ1 and BMP2 pro-tein of cartilage and synovial of OA.
